SITEIA.PARMA Interdepartmental Centre, University of Parma, Parco Area Delle Scienze 181/A, 43124 Parma, Italy.
Food Chem. 2013 Dec 15;141(4):3820-6. doi: 10.1016/j.foodchem.2013.06.075. Epub 2013 Jun 28.
Extra virgin olive oil is frequently subjected to adulterations with addition of oils obtained from plants other than olive. DNA analysis is a fast and economic tool to identify plant components in oils. Extraction and amplification of DNA by PCR was tested in olives, in milled seeds and in oils, to investigate its use in olive oil traceability. DNA was extracted from different oils made of hazelnut, maize, sunflower, peanut, sesame, soybean, rice and pumpkin. Comparing the DNA melting profiles in reference plant materials and in the oils, it was possible to identify any plant components in oils and mixtures of oils. Real-Time PCR (RT-PCR) platform has been added of the new methodology of high resolution melting (HRM), both were used to analyse olive oils mixed with different percentage of other oils. Results showed HRM a cost effective method for efficient detection of adulterations in olive oils.
特级初榨橄榄油经常被掺入其他植物来源的油。DNA 分析是一种快速且经济的工具，可用于鉴定油中的植物成分。本文测试了从橄榄、磨碎的种子和油中提取和扩增 DNA 的方法，以研究其在橄榄油可追溯性中的应用。从榛子、玉米、葵花籽、花生、芝麻、大豆、大米和南瓜制成的不同油中提取了 DNA。比较参考植物材料和油中的 DNA 熔解曲线，可鉴定油和油混合物中的任何植物成分。将实时 PCR（RT-PCR）平台添加到高分辨率熔解（HRM）的新方法中，均用于分析与不同比例其他油混合的橄榄油。结果表明 HRM 是一种经济有效的方法，可有效检测橄榄油中的掺假。
