Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, n. 9500, CEP 91501-970, Porto Alegre, RS, Brazil.
Food Chem. 2013 Dec 15;141(4):3906-12. doi: 10.1016/j.foodchem.2013.04.135. Epub 2013 Jun 19.
The aim of this study was to produce bixin nanocapsules by the interfacial deposition of preformed poly-ε-caprolactone (PCL). PCL (250 mg), capric/caprylic triglyceride (400 μL), sorbitan monostearate (95 mg) and bixin were dissolved in a mixture of acetone (60 mL) and ethanol (7.5 mL) under stirring (40 °C). This organic solution was added to the aqueous solution (130 mL) containing Tween 80 (195 mg). The size distributions in the formulations with bixin concentration from 11 to 100 μg/mL were evaluated periodically during 3 weeks of storage at ambient temperature. The optimal formulation (bixin concentration of 16.92±0.16 μg/mL) was characterised in terms of particle size distribution, zeta potential, bixin content and encapsulation efficiency, and showed a volume-weighted mean diameter (D4,3) of 195±27 nm, around 100% of encapsulation efficiency and the nanocapsules were considered physically stable during 119 days of storage at ambient temperature.
本研究旨在通过界面沉积法制备聚己内酯（PCL）纳米胶囊。将 PCL（250mg）、辛酸/癸酸三甘油酯（400μL）、山梨醇单硬脂酸酯（95mg）和胭脂树橙溶解于丙酮（60mL）和乙醇（7.5mL）的混合溶液中，在搅拌（40°C）下进行。将该有机溶剂溶液加入含有吐温 80（195mg）的水溶液（130mL）中。在室温下储存期间，定期评估含有胭脂树橙浓度为 11 至 100μg/mL 的制剂的粒径分布。最优制剂（胭脂树橙浓度为 16.92±0.16μg/mL）的粒径分布、Zeta 电位、胭脂树橙含量和包封效率进行了表征，其体积加权平均直径（D4,3）为 195±27nm，包封效率接近 100%，在室温下储存 119 天期间被认为是物理稳定的。
