Kapustová Magdaléna, Granata Giuseppe, Napoli Edoardo, Puškárová Andrea, Bučková Mária, Pangallo Domenico, Geraci Corrada
Institute of Molecular Biology, Slovak Academy of Sciences, Dúbravská cesta 21, 84551 Bratislava, Slovakia.
Istituto Chimica Biomolecolare-Consiglio Nazionale delle Ricerche, Via Paolo Gaifami 18, 95126 Catania, Italy.
Antibiotics (Basel). 2021 Jan 1;10(1):31. doi: 10.3390/antibiotics10010031.
Nanotechnology is a new frontier of this century that finds applications in various fields of science with important effects on our life and on the environment. Nanoencapsulation of bioactive compounds is a promising topic of nanotechnology. The excessive use of synthetic compounds with antifungal activity has led to the selection of resistant fungal species. In this context, the use of plant essential oils (EOs) with antifungal activity encapsulated in ecofriendly nanosystems could be a new and winning strategy to overcome the problem. We prepared nanoencapsules containing the essential oils of (OV) and (TC) by the nanoprecipitation method. The colloidal suspensions were characterized for size, polydispersity index (PDI), zeta potential, efficiency of encapsulation (EE) and loading capacity (LC). Finally, the essential oil nanosuspensions were assayed against a panel of fourteen fungal strains belonging to the Ascomycota and Basidiomycota phyla. Our results show that the nanosystems containing thyme and oregano essential oils were active against various fungal strains from natural environments and materials. In particular, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values were two to four times lower than the pure essential oils. The aqueous, ecofriendly essential oil nanosuspensions with broad-spectrum antifungal activity could be a valid alternative to synthetic products, finding interesting applications in the agri-food and environmental fields.
纳米技术是本世纪的一个新前沿领域，在科学的各个领域都有应用，对我们的生活和环境有着重要影响。生物活性化合物的纳米包封是纳米技术中一个很有前景的课题。具有抗真菌活性的合成化合物的过度使用导致了抗真菌物种的产生。在这种情况下，将具有抗真菌活性的植物精油（EOs）封装在生态友好型纳米系统中可能是解决这一问题的一种新的成功策略。我们通过纳米沉淀法制备了含有牛至（OV）和百里香（TC）精油的纳米胶囊。对胶体悬浮液的粒径、多分散指数（PDI）、zeta电位、包封效率（EE）和载药量（LC）进行了表征。最后，对一组属于子囊菌门和担子菌门的14种真菌菌株进行了精油纳米悬浮液的检测。我们的结果表明，含有百里香和牛至精油的纳米系统对来自自然环境和材料的各种真菌菌株具有活性。特别是，最低抑菌浓度（MIC）和最低杀菌浓度（MFC）值比纯精油低两到四倍。具有广谱抗真菌活性的水性、生态友好型精油纳米悬浮液可能是合成产品的有效替代品，在农业食品和环境领域有有趣的应用。
