Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Tissue Cell. 2013 Dec;45(6):421-7. doi: 10.1016/j.tice.2013.07.005. Epub 2013 Aug 29.
GPR120 functions as a plasma membrane receptor for unsaturated long-chain free fatty acids and involves in GLP-1 secretion, adipogenesis and the control of energy balance. Pancreas is the key organ in fuel and energy metabolism. Here GPR120 expression in human and rat pancreas was observed by RT-PCR, and the distribution and phenotypes of GPR120-positive cells in human and rat pancreas were shown by immunohistochemical staining. GPR120 mRNA expression was found in human and rat pancreas. GPR120-positive cells were scattered mainly in the interstitial tissues of human and rat pancreas, and they were not co-localized with nestin, vimentin, alpha-SMA and glucagon, respectively. However, GPR120 was distributed on the cells positively stained by CD68, the specific marker of macrophages, and on the cells positive stained by CD34 and CD117, the markers of interstitial cells. In conclusion, this study demonstrates the expression of GPR120 in pancreas and shows the distribution of GPR120 in human and rat pancreas.
GPR120 作为一种位于质膜上的受体，可与不饱和长链游离脂肪酸结合，参与 GLP-1 的分泌、脂肪生成和能量平衡的调控。胰腺是参与能量代谢和物质代谢的重要器官。本研究通过 RT-PCR 观察了 GPR120 在人及大鼠胰腺中的表达，通过免疫组化染色观察了 GPR120 阳性细胞在人及大鼠胰腺中的分布和表型。结果显示，GPR120 在人及大鼠胰腺中均有表达。GPR120 阳性细胞主要分布于人及大鼠胰腺的间质组织中，且分别与巢蛋白、波形蛋白、α-SMA 和胰高血糖素无共定位。然而，GPR120 分布于 CD68（巨噬细胞的特异性标志物）阳性细胞以及 CD34 和 CD117（间质细胞标志物）阳性细胞上。综上所述，本研究证实了 GPR120 在胰腺中的表达，并展示了 GPR120 在人及大鼠胰腺中的分布。
