Jia Shaoyi, Li Feng, Liu Yong, Ren Haitao, Gong Guili, Wang Yanyan, Wu Songhai
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
Int J Biol Macromol. 2013 Nov;62:66-9. doi: 10.1016/j.ijbiomac.2013.08.031. Epub 2013 Aug 28.
Five polysaccharides were obtained from Agaricus blazei Murrill (ABM) through different extraction methods including hot water extraction, single enzyme extraction (pectinase, cellulase or papain) and compound enzymes extraction (cellulase:pectinase:papain). Their characteristics such as the polysaccharide yield, polysaccharide content, protein content, infrared spectra were determined, and antioxidant activities were investigated on the basis of hydroxyl radical, DPPH free radical, ABTS free radical and reducing power. The results showed that five extracts exhibited antioxidant activities in a concentration-dependent manner. Compared with other methods, the compound enzymes extraction method was found to present the highest polysaccharides yield (17.44%). Moreover, compound enzymes extracts exhibited the strongest reducing power and highest scavenging rates on hydroxyl radicals, DPPH radicals and ABTS radicals. On the contrary, hot water extraction method had the lowest polysaccharides yield of 11.95%, whose extracts also exhibited the lowest antioxidant activities. Overall, the available data obtained in vitro models suggested that ABM extracts were natural antioxidants and compound enzymes extraction was an appropriate, mild and effective extracting method for obtaining the polysaccharide extracts from Agaricus blazei Murrill (ABM).
通过不同提取方法从姬松茸中获得了五种多糖，这些方法包括热水提取、单一酶提取（果胶酶、纤维素酶或木瓜蛋白酶）和复合酶提取（纤维素酶:果胶酶:木瓜蛋白酶）。测定了它们的多糖得率、多糖含量、蛋白质含量、红外光谱等特性，并基于羟基自由基、DPPH自由基、ABTS自由基和还原力研究了其抗氧化活性。结果表明，五种提取物均呈现出浓度依赖性的抗氧化活性。与其他方法相比，复合酶提取法的多糖得率最高（17.44%）。此外，复合酶提取物表现出最强的还原力以及对羟基自由基、DPPH自由基和ABTS自由基的最高清除率。相反，热水提取法的多糖得率最低，为11.95%，其提取物的抗氧化活性也最低。总体而言，体外模型获得的现有数据表明，姬松茸提取物是天然抗氧化剂，复合酶提取是从姬松茸中获得多糖提取物的一种合适、温和且有效的提取方法。
