School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, PR China.
Carbohydr Polym. 2014 Mar 15;103:414-7. doi: 10.1016/j.carbpol.2013.11.075. Epub 2013 Dec 25.
Three polysaccharides (ABMP-F, ABMP-V, ABMP-A) were obtained from Agaricus blazei Murrill via methods such as freeze drying, vacuum drying and air drying, respectively. Their chemical compositions were examined, and antioxidant activities were investigated on the basis of assay for hydroxyl radical, DPPH radical, ABTS free radical scavenging ability and assay for Fe(2+)-chelating ability. Results showed that the three ABMPs have different physicochemical and antioxidant properties. Compared with air drying and vacuum drying methods, freeze drying method resulted to ABMP with higher neutral sugar, polysaccharide yield, uronic acid content, and stronger antioxidant abilities of hydroxyl radical, DPPH radical, ABTS radical scavenging and Fe(2+)-chelating. As a result, Agaricus blazei Murrill polysaccharides are natural antioxidant and freeze drying method serves as a good choice for the preparation of such polysaccharides and should be used to produce antioxidants for food industry.
三种多糖（ABMP-F、ABMP-V、ABMP-A）分别通过冷冻干燥、真空干燥和空气干燥等方法从巴西蘑菇中提取得到。对其化学成分进行了检测，并基于羟基自由基、DPPH 自由基清除能力、ABTS 自由基清除能力和 Fe（2+）螯合能力测定，研究了其抗氧化活性。结果表明，三种 ABMP 具有不同的理化性质和抗氧化活性。与空气干燥和真空干燥方法相比，冷冻干燥法得到的 ABMP 具有更高的中性糖、多糖得率、糖醛酸含量，以及更强的羟基自由基、DPPH 自由基、ABTS 自由基清除和 Fe（2+）螯合能力。因此，巴西蘑菇多糖是天然的抗氧化剂，冷冻干燥法是制备此类多糖的良好选择，可用于食品工业生产抗氧化剂。
