Departments of Anatomy, Weifang Medical University, Weifang 261053, China.
Int J Mol Sci. 2013 Aug 29;14(9):17680-93. doi: 10.3390/ijms140917680.
The purpose of this study was to investigate the possible protective effect of N-acetylserotonin (NAS) against acute hepatic ischemia-reperfusion (I/R) injury in mice. Adult male mice were randomly divided into three groups: sham, I/R, and I/R + NAS. The hepatic I/R injury model was generated by clamping the hepatic artery, portal vein, and common bile duct with a microvascular bulldog clamp for 30 min, and then removing the clamp and allowing reperfusion for 6 h. Morphologic changes and hepatocyte apoptosis were evaluated by hematoxylin-eosin (HE) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Activated caspase-3 expression was evaluated by immunohistochemistry and Western blot. The activation of aspartate aminotransferase (AST), malondialdehyde (MDA), and superoxide dismutase (SOD) was evaluated by enzyme-linked immunosorbent assay (ELISA). The data show that NAS rescued hepatocyte morphological damage and dysfunction, decreased the number of apoptotic hepatocytes, and reduced caspase-3 activation. Our work demonstrates that NAS ameliorates hepatic IR injury.
本研究旨在探讨 N-乙酰血清素（NAS）对小鼠急性肝缺血再灌注（I/R）损伤的可能保护作用。成年雄性小鼠随机分为三组：假手术组、I/R 组和 I/R+NAS 组。通过用微血管止血夹夹闭肝动脉、门静脉和胆总管 30 分钟来建立肝 I/R 损伤模型，然后松开止血夹并允许再灌注 6 小时。通过苏木精-伊红（HE）和末端脱氧核苷酸转移酶 dUTP 缺口末端标记（TUNEL）染色分别评估形态变化和肝细胞凋亡。通过免疫组织化学和 Western blot 评估激活的 caspase-3 表达。通过酶联免疫吸附试验（ELISA）评估天门冬氨酸氨基转移酶（AST）、丙二醛（MDA）和超氧化物歧化酶（SOD）的活性。数据表明，NAS 挽救了肝细胞的形态损伤和功能障碍，减少了凋亡肝细胞的数量，并降低了 caspase-3 的激活。我们的工作表明，NAS 改善了肝 I/R 损伤。
