Department of Forage Germplasm Resources, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China.
Plant Physiol Biochem. 2013 Dec;73:1-6. doi: 10.1016/j.plaphy.2013.07.015. Epub 2013 Aug 6.
Gibberellins (GAs) are well-known phytohormones that contribute to a wide range of plant growth and development functions including stem elongation and leaf expansion. GA receptors perceive GA and transmit signals to activate GA-regulated reactions. In this study, a GA receptor gene with homology to other leguminous plants was isolated from Galega orientalis and termed GoGID. The 1732-bp full-length GoGID gene included an open reading frame of 1035 bp encoding a peptide of 344 amino acids. Sequence analysis indicated that GoGID shares conserved HGGS motif and active amino acid sites (Ser-Asp-Val/IIe) that are essential for maintaining it GA-binding activity. GoGID mRNA expression was more abundant in leaves than in roots or stems and could be up-regulated by the exogenous hormones. Overexpression of GoGID in transgenic tobacco plants promoted plant elongation and improved biomass production. These results suggested that GoGID functions as a GA receptor to alter GA-mediated signaling. GoGID may have a role in genetic engineering for the improvement of forage crops.
赤霉素（GAs）是一种众所周知的植物激素，参与多种植物生长和发育功能，包括茎伸长和叶片扩张。GA 受体感知 GA 并传递信号以激活 GA 调节的反应。本研究从山羊豆中分离出与其他豆科植物同源的 GA 受体基因，并将其命名为 GoGID。GoGID 的全长 1732bp 基因包括一个 1035bp 的开放阅读框，编码一个由 344 个氨基酸组成的肽。序列分析表明，GoGID 共享保守的 HGGS 基序和活性氨基酸位点（Ser-Asp-Val/IIe），这些位点对于维持其 GA 结合活性是必不可少的。GoGID 在叶片中的表达量高于根和茎，并且可以被外源激素上调。GoGID 在转基因烟草植株中的过表达促进了植物的伸长并提高了生物量的产生。这些结果表明，GoGID 作为 GA 受体发挥作用，改变 GA 介导的信号转导。GoGID 可能在牧草作物遗传改良的基因工程中发挥作用。
