Du Dengfeng, Gao Xin, Geng Juan, Li Qingyan, Li Liqun, Lv Qian, Li Xuejun
State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University Yangling, China.
Front Plant Sci. 2016 Jun 28;7:922. doi: 10.3389/fpls.2016.00922. eCollection 2016.
In wheat, coding region allelic variants of TaGW2-6A are closely associated with grain width and weight, but the genetic mechanisms involved remain unclear. Thus, to obtain insights into the key functions regulated by TaGW2-6A during wheat grain development, we performed transcriptional and proteomic analyses of TaGW2-6A allelic variants. The transcription results showed that the TaGW2-6A allelic variants differed significantly by several orders of magnitude. Each allelic variant of TaGW2-6A reached its first transcription peak at 6 days after anthesis (DAA), but the insertion type TaGW2-6A allelic variant reached its second peak earlier than the normal type, i.e., at 12 DAA rather than 20 DAA. In total, we identified 228 differentially accumulated protein spots representing 138 unique proteins by two-dimensional gel electrophoresis and tandem MALDI-TOF/TOF-MS in these three stages. Based on the results, we found some key proteins that are closely related to wheat grain development. The results of this analysis improve our understanding of the genetic mechanisms related to TaGW2-6A during wheat grain development as well as providing insights into the biological processes involved in seed formation.
在小麦中，TaGW2-6A的编码区等位变异与籽粒宽度和重量密切相关，但其涉及的遗传机制仍不清楚。因此，为了深入了解TaGW2-6A在小麦籽粒发育过程中调控的关键功能，我们对TaGW2-6A等位变异进行了转录组和蛋白质组分析。转录结果表明，TaGW2-6A等位变异在几个数量级上存在显著差异。TaGW2-6A的每个等位变异在开花后6天（DAA）达到第一个转录峰值，但插入型TaGW2-6A等位变异比正常型更早达到第二个峰值，即在12 DAA而不是20 DAA。在这三个阶段，我们通过二维凝胶电泳和串联MALDI-TOF/TOF-MS总共鉴定出228个差异积累的蛋白质点，代表138种独特的蛋白质。基于这些结果，我们发现了一些与小麦籽粒发育密切相关的关键蛋白质。该分析结果提高了我们对小麦籽粒发育过程中与TaGW2-6A相关的遗传机制的理解，同时也为种子形成所涉及的生物学过程提供了见解。
