Institute of Chemistry, Federal University of Goiás, Campus Samambaia, Caixa Postal 131, Goiânia, Goiás CEP 74001-970, Brazil.
Org Biomol Chem. 2013 Oct 21;11(39):6695-8. doi: 10.1039/c3ob41318g. Epub 2013 Sep 2.
Evidence is provided for the inner-sphere mechanism with actual metal coordination of the racemic amine in the crucial hydrogen transfer step promoted by Shvo's catalyst of the chemoenzymatic dynamic kinetic resolution (DKR) of amines. Key intermediates involved in this H-transfer step were intercepted and continuously monitored by electrospray ionization mass spectrometry (ESI-MS) and characterized by their dissociation chemistries via ESI-MS/MS.
为 Shvo 催化剂促进的手性胺的化学酶动力学动态拆分（DKR）中关键的氢转移步骤中，手性胺实际的金属配位的内球机制提供了证据。通过电喷雾电离质谱（ESI-MS）拦截并连续监测该 H 转移步骤中涉及的关键中间体，并通过 ESI-MS/MS 研究其解离化学对其进行了表征。
