Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str, Zografou Campus, 15700, Athens, Greece.
Appl Microbiol Biotechnol. 2013 Oct;97(19):8455-65. doi: 10.1007/s00253-013-5197-y. Epub 2013 Aug 31.
The classical hydrolytic mechanism for the degradation of plant polysaccharides by saprophytic microorganisms has been reconsidered after the recent landmark discovery of a new class of oxidases termed lytic polysaccharide monooxygenases (LPMOs). LPMOs are of increased biotechnological interest due to their implication in lignocellulosic biomass decomposition for the production of biofuels and high-value chemicals. They act on recalcitrant polysaccharides by a combination of hydrolytic and oxidative function, generating oxidized and non-oxidized chain ends. They are copper-dependent and require molecular oxygen and an external electron donor for their proper function. In this review, we present the recent findings concerning the mechanism of action of these oxidative enzymes and identify issues and questions to be addressed in the future.
最近，人们发现了一类新的氧化酶，称为溶菌多糖单加氧酶（LPMO），这使得对腐生微生物降解植物多糖的经典水解机制的重新思考。由于它们在木质纤维素生物质分解方面的作用，对于生产生物燃料和高价值化学品具有重要的生物技术意义，因此 LPMO 引起了人们越来越多的关注。它们通过水解和氧化功能的结合作用于抗性多糖，生成氧化和非氧化的链末端。它们依赖铜，并需要分子氧和外部电子供体才能正常发挥作用。在这篇综述中，我们介绍了这些氧化酶的作用机制的最新发现，并确定了未来需要解决的问题和疑问。
