Institute of Computer Science and Department of Genetics, Institute of Biology, Martin Luther University Halle-Wittenberg, D-06099 Halle (Saale), Germany.
Bioinformatics. 2013 Nov 15;29(22):2931-2. doi: 10.1093/bioinformatics/btt501. Epub 2013 Aug 30.
Transcription activator-like effector nucleases (TALENs) have become an accepted tool for targeted mutagenesis, but undesired off-targets remain an important issue. We present TALENoffer, a novel tool for the genome-wide prediction of TALEN off-targets. We show that TALENoffer successfully predicts known off-targets of engineered TALENs and yields a competitive runtime, scanning complete mammalian genomes within a few minutes.
TALENoffer is available as a command line program from http://www.jstacs.de/index.php/TALENoffer and as a Galaxy server at http://galaxy.informatik.uni-halle.de.
转录激活因子样效应物核酸酶（TALENs）已成为一种用于靶向诱变的可接受工具，但非预期的脱靶仍然是一个重要问题。我们介绍了 TALENoffer，这是一种用于全基因组预测 TALEN 脱靶的新型工具。我们表明，TALENoffer 成功预测了工程 TALEN 的已知脱靶，并具有竞争力的运行时间，在几分钟内扫描完整的哺乳动物基因组。
TALENoffer 可作为命令行程序从 http://www.jstacs.de/index.php/TALENoffer 获得，并作为 Galaxy 服务器从 http://galaxy.informatik.uni-halle.de 获得。
