Quan Gui-Hua, Chae Hee-Sung, Song Hyuk Hwan, Ahn Kyoung-Seop, Lee Hyeong-Kyu, Kim Young-Ho, Oh Sei-Ryang, Chin Young-Won
Central Research Laboratory, The Second Clinical Hospital of Jilin University.
Chem Pharm Bull (Tokyo). 2013;61(9):920-6. doi: 10.1248/cpb.c13-00239.
Bioactivity-guided fractionation for an EtOAc-soluble fraction of methanolic extract of Arthraxon hispidus, using primary cell assay with bone marrow-derived mast cells (BMMC), led to an isolation of six new flavones and nine known compounds. The structures of the new compounds were established by one dimensional (1D)- and 2D-NMR spectroscopic data, as luteolin 8-C-β-kerriopyranoside (1), luteolin 8-acetic acid methyl ester (2), 7-methyl-luteolin 8-C-β-(6-deoxyxylo-3-uloside) (3), apigenin 8-C-α-fucopyranoside (4), apigenin 8-C-β-fucopyranoside (5) and luteolin 8-C-β-fucopyranoside (6). All the isolates were evaluated for inhibitory activities on interleukin-6 release in the primary cultures using BMMC. Of the tested compounds, compounds 2, 3 and 10 were found to inhibit interleukin-6 release. Furthermore, compound 2 displayed inhibitory activity against prostaglandin D2, leukotriene C4, and β-hexosaminidase releases.
采用骨髓来源的肥大细胞（BMMC）原代细胞分析法，对荩草甲醇提取物的乙酸乙酯可溶部分进行生物活性导向分离，得到了6种新黄酮和9种已知化合物。通过一维（1D）和二维（2D）核磁共振光谱数据确定了新化合物的结构，分别为木犀草素8-C-β-克里奥吡喃糖苷（1）、木犀草素8-乙酸甲酯（2）、7-甲基木犀草素8-C-β-（6-脱氧木糖-3-酮糖苷）（3）、芹菜素8-C-α-岩藻吡喃糖苷（4）、芹菜素8-C-β-岩藻吡喃糖苷（5）和木犀草素8-C-β-岩藻吡喃糖苷（6）。使用BMMC对所有分离物在原代培养物中抑制白细胞介素-6释放的活性进行了评估。在测试的化合物中，发现化合物2、3和10能抑制白细胞介素-6的释放。此外，化合物2对前列腺素D2、白三烯C4和β-己糖胺酶的释放具有抑制活性。
