Nephrology and Dialysis Department, I.R.C.C.S. "Casa Sollievo della Sofferenza", S. Giovanni Rotondo (Foggia), Italy.
J Pharm Biomed Anal. 2013 Dec;86:123-6. doi: 10.1016/j.jpba.2013.08.001. Epub 2013 Aug 11.
Kidney transplanted patients need immunosuppressant therapies. Therapeutic drug monitoring approaches produce the optimal clinical outcome is still under debate. This review details strength and limits of methods available: immunoassay and chromatography-based. Liquid chromatography with mass spectrometry detection is a major breakthrough in therapeutic drug monitoring of immunosuppressive agents and is considered as the method of choice in therapeutic drug monitoring (TDM) of immunosuppressants. Despite the initial high cost for the instrumentation, HPLC-MS is more cost effective than microparticle enzyme immunoassay. The main important features of LC-MS/MS methodology for immunosuppressive drugs are the shortened analysis time, an increased throughput, higher selectivity, specificity, and sensitivity, and low cost of analysis.
肾移植患者需要免疫抑制剂治疗。治疗药物监测方法能否产生最佳的临床效果仍存在争议。本综述详细介绍了现有的方法：免疫测定法和基于色谱法的优缺点。液质联用技术在免疫抑制剂治疗药物监测方面取得了重大突破，被认为是免疫抑制剂治疗药物监测（TDM）的首选方法。尽管仪器的初始成本较高，但 HPLC-MS 比微粒子酶免疫测定法更具成本效益。LC-MS/MS 方法学用于免疫抑制剂的主要重要特征是缩短了分析时间、提高了通量、更高的选择性、特异性和灵敏度，以及更低的分析成本。
