Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
Chem Commun (Camb). 2013 Oct 14;49(80):9203-5. doi: 10.1039/c3cc45750h. Epub 2013 Aug 30.
We report a simple, direct fluorometric assay based on graphene oxide (GO) for RNA polymerase-mediated RNA synthesis. In principle, fluorescent peptide nucleic acid (PNA) probes were designed, and annealed with RNA products and the resultant RNA-PNA hybrids induced the recovery of fluorescence intensity of the PNA probes adsorbed onto the GO surface.
我们报道了一种基于氧化石墨烯（GO）的简单、直接的荧光分析方法，用于 RNA 聚合酶介导的 RNA 合成。从原理上讲，设计了荧光肽核酸（PNA）探针，并与 RNA 产物退火，所得的 RNA-PNA 杂交体诱导吸附在 GO 表面上的 PNA 探针的荧光强度恢复。
