一种基于聚合酶链式反应（PCR）的荧光检测系统，用于使用无淬灭剂荧光DNA探针和氧化石墨烯检测突变融合DNA。
A simple PCR-based fluorometric system for detection of mutant fusion DNAs using a quencher-free fluorescent DNA probe and graphene oxide.
作者信息
Roh Kyoungmin, Kim Dong-Min, Lee Eun Hee, Kim Hyoseon, Park Hyung Soon, Jang Ja-Hyun, Hwang Sang-Hyun, Kim Dong-Eun
机构信息
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
出版信息
Chem Commun (Camb). 2015 Apr 25;51(32):6960-3. doi: 10.1039/c5cc00263j. Epub 2015 Mar 23.
PMID:25797656
Abstract
We propose a facile fluorometric system for detection of gene mutations using graphene oxide (GO). A fluorescent probe DNA anneals to a specific mutant gene and is degraded by the 5'→ 3' exonuclease activity of Taq polymerase during PCR, and the released fluorophore retains fluorescence after addition of GO without quenching.
摘要
我们提出了一种使用氧化石墨烯（GO）检测基因突变的简易荧光系统。一种荧光探针DNA与特定的突变基因退火，并在PCR过程中被Taq聚合酶的5'→3'核酸外切酶活性降解，释放出的荧光团在加入GO后保持荧光而不被淬灭。
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