利用氧化石墨烯和脱氧核酶基于荧光检测RNA中的单核苷酸变化
Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme.
作者信息
Hong Chaesun, Kim Dong-Min, Baek Ahruem, Chung Hyewon, Jung Woong, Kim Dong-Eun
机构信息
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
出版信息
Chem Commun (Camb). 2015 Apr 4;51(26):5641-4. doi: 10.1039/c4cc09603g.
PMID:25714982
Abstract
We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.
摘要
我们报道了一种使用氧化石墨烯（GO）和RNA切割型脱氧核酶检测RNA中单个核苷酸变化的简单荧光测定方法。荧光DNA探针（F-DNA）与由针对突变RNA的脱氧核酶切割RNA产生的RNA片段退火。F-DNA-RNA双链体减弱了GO对F-DNA荧光的淬灭作用。
Zotero 插件