Barnes Sophie C
Imperial College Healthcare NHS Trust, Charing Cross Hospital, London, UK.
Methods Mol Biol. 2013;1065:227-34. doi: 10.1007/978-1-62703-616-0_14.
This chapter describes the measurement of aldosterone concentration in plasma or serum. Aldosterone specificity is improved by first extracting the aldosterone into dichloromethane. A radioimmunoassay is then performed on the dried and reconstituted extract using an anti-aldosterone antibody and iodinated aldosterone. The antibody-bound fraction is separated from the unbound fraction using activated charcoal. The notes section provides extra information for steps in the assay that can be problematic.
本章介绍了血浆或血清中醛固酮浓度的测定方法。首先将醛固酮提取到二氯甲烷中，以提高醛固酮的特异性。然后使用抗醛固酮抗体和碘化醛固酮对干燥并复溶的提取物进行放射免疫分析。使用活性炭将结合抗体的部分与未结合的部分分离。注释部分为分析过程中可能出现问题的步骤提供了额外信息。
