Priya S, Nithya R, Berchmans Sheela
Electrodics and Electrocatalysis Division, Central Electrochemical Research Institute, Karaikudi, 630006, Tamil Nadu, India,
J Mater Sci Mater Med. 2014 Jan;25(1):1-10. doi: 10.1007/s10856-013-5032-0. Epub 2013 Aug 31.
In this work, we portray a new controlled nitric oxide (NO) delivery platform by grafting S-nitrosothiol derived from cysteine into the polymeric backbone of poly(vinyl methyl ether-co-maleic anhydride). Nitrosothiols (RSNO's) are linked to the polymeric backbone through solvent displacement method. By adjusting solvent polarity, materials of different shapes and sizes varying between μm and nm are prepared. More often our method of preparation resulted in hexagonally shaped polymeric materials. The structure and RSNO conjugation analysis was investigated using scanning electron microscopy (SEM), FT-IR, UV-Vis spectroscopy and thermogravimetric analysis (TGA). Bactericidal efficacy of nitric oxide releasing polymer hexagons, a novel antibacterial agent is demonstrated against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Confocal microscopic studies revealed the enhanced bactericidal effect of polymer hexagons via membrane destruction. Results suggest that this biocompatible NO releasing RSNO conjugated polymer hexagons could be potentially useful for antimicrobial applications.
在这项工作中，我们通过将源自半胱氨酸的S-亚硝基硫醇接枝到聚（乙烯基甲基醚-马来酸酐）的聚合物主链中，描绘了一种新的可控一氧化氮（NO）递送平台。亚硝基硫醇（RSNO）通过溶剂置换法连接到聚合物主链上。通过调节溶剂极性，制备了形状和尺寸在微米和纳米之间变化的不同材料。我们的制备方法更常得到六边形的聚合物材料。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、紫外可见光谱（UV-Vis）和热重分析（TGA）研究了结构和RSNO共轭分析。释放一氧化氮的聚合物六边形作为一种新型抗菌剂，对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌具有杀菌效果。共聚焦显微镜研究揭示了聚合物六边形通过膜破坏增强的杀菌作用。结果表明，这种具有生物相容性的释放NO的RSNO共轭聚合物六边形可能在抗菌应用中具有潜在用途。
