Rho-GDP 解离抑制剂-γ 的过表达抑制神经干细胞的迁移。

Overexpression of Rho-GDP-dissociation inhibitor-γ inhibits migration of neural stem cells.

机构信息

Laboratory of Molecular Neural Biology, Institute of Systems Biology, School of Life Sciences, Shanghai University, Shanghai, China; School of Communication and Information Engineering, Shanghai University, Shanghai, China.

出版信息

J Neurosci Res. 2013 Nov;91(11):1394-401. doi: 10.1002/jnr.23261. Epub 2013 Aug 30.

DOI:10.1002/jnr.23261

PMID:23996536

Abstract

Neural stem cell (NSC) migration relies heavily on the regulation of actin and microtubule cytoskeletons by Rho GTPases, which are critical regulators of key steps during NSC migration. However, the migration mechanism remains unclear. Rho-GDP-dissociation inhibitor-γ (Rho-GDIγ) was identified as an important downregulator of the Rho family of GTPases, because of its ability to prevent nucleotide exchange and thus membrane association. This study investigates the role of Rho-GDIγ in neural stem cells migration. Our results indicate that the overexpression of Rho-GDIγ maintains NSCs in the stem cell state, meanwhile preventing NSC migration through inhibition of Rac1 expression, one of the Rho-family GTPases. This study provides the basis for further study of the molecular mechanism of NSC migration.

摘要

神经干细胞（NSC）的迁移在很大程度上依赖于 Rho GTPases 对肌动蛋白和微管细胞骨架的调节，Rho GTPases 是 NSC 迁移过程中关键步骤的关键调节剂。然而，迁移机制尚不清楚。Rho-GDP 解离抑制剂-γ（Rho-GDIγ）被鉴定为 Rho 家族 GTPases 的重要下调因子，因为它能够阻止核苷酸交换，从而阻止膜结合。本研究探讨了 Rho-GDIγ 在神经干细胞迁移中的作用。我们的结果表明，Rho-GDIγ 的过表达使 NSCs 保持在干细胞状态，同时通过抑制 Rac1 表达来阻止 NSC 迁移，Rac1 是 Rho 家族 GTPases 之一。本研究为进一步研究 NSC 迁移的分子机制提供了基础。