Lu Wei, Wang Jiao, Wen Tieqiao
Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, 99 Shang Da Road, Shanghai 200444, China.
Mol Cell Biochem. 2008 Apr;311(1-2):233-40. doi: 10.1007/s11010-008-9713-9. Epub 2008 Feb 14.
Rho-GDIgamma belongs to the Rho-GDI protein family, which was observed to have high level expression in the entire brain. Although it exists in neuronal population, its physiological function is poorly understood. This study shows that Rho-GDIgamma is a key factor in the G13 signaling pathway based on an analysis of global gene expression. By using RNAi technology to downregulate expression of Rho-GDIgamma we found distinct morphological changes in neural stem cell line C17.2. More important, RT-PCR confirmed that RNAi-mediated downregulation of Rho-GDIgamma decreased expression of Rho-GDIgamma-regulated genes RhoA, Cdc42, Limk2, and N-WASP and slightly increased expression of Rac1. Further, immunochemical staining indicated that downregulation of Rho-GDIgamma increased the tendency of C17.2 cells to differentiate. These data strongly suggest that Rho-GDIgamma plays a key role in the differentiation of neural stem cells.
Rho-GDIγ属于Rho-GDI蛋白家族，在整个大脑中呈现高水平表达。尽管它存在于神经元群体中，但其生理功能仍知之甚少。基于对整体基因表达的分析，本研究表明Rho-GDIγ是G13信号通路中的关键因子。通过使用RNA干扰技术下调Rho-GDIγ的表达，我们发现神经干细胞系C17.2出现了明显的形态变化。更重要的是，逆转录-聚合酶链反应证实，RNA干扰介导的Rho-GDIγ下调降低了Rho-GDIγ调控基因RhoA、Cdc42、Limk2和N-WASP的表达，并略微增加了Rac1的表达。此外，免疫化学染色表明，Rho-GDIγ的下调增加了C17.2细胞的分化倾向。这些数据有力地表明，Rho-GDIγ在神经干细胞的分化中起关键作用。
