Pan Xin-Min, Jia Jing, Guo Xiao-Min, Li Zhao-Hui, Zhang Zhen, Qin Hao-Jie, Xu Guo-Hui, Gao Lin-Bo
Department of Forensic Pathology, College of Forensic Medicine, Henan University of Science and Technology, Luoyang, 471003, Henan, People's Republic of China,
Fam Cancer. 2014 Mar;13(1):93-7. doi: 10.1007/s10689-013-9681-4.
Nasopharyngeal carcinoma (NPC) is characterized by its highly invasive and metastatic features. Therefore, screening genetic biomarkers of NPC to achieve early diagnose would be of great value for NPC therapy. Single nucleotide polymorphisms in let-7 miRNA binding site in 3' untranslated region of KRAS mRNA have been found to be associated with various cancer risks. In this study, we genotyped the frequency of KRAS rs712 to test its effect on NPC risk in a hospital-based case-control study in a Chinese population, with 188 histologically confirmed NPC patients and 356 cancer-free controls, using polymerase chain reaction-restriction fragment length polymorphism assay. There was no significant difference in the genotype and allele frequencies of the rs712 polymorphism between the NPC patients and the control group (GT vs. GG, OR 0.83, 95% CI 0.57-1.21; TT vs. GG, OR 1.27, 95% CI 0.58-2.75). Our data suggest that the KRAS rs712 polymorphism in let-7 miRNA binding site has no association with NPC risk. Further experiments with larger sample size or other polymorphism sites are needed to verify the result, especially in different ethnic groups.
鼻咽癌（NPC）具有高度侵袭性和转移性的特征。因此，筛查鼻咽癌的遗传生物标志物以实现早期诊断对鼻咽癌治疗具有重要价值。已发现KRAS mRNA 3'非翻译区中let-7 miRNA结合位点的单核苷酸多态性与多种癌症风险相关。在本研究中，我们对KRAS rs712的频率进行基因分型，以在中国人群的一项基于医院的病例对照研究中测试其对鼻咽癌风险的影响，该研究纳入了188例经组织学确诊的鼻咽癌患者和356例无癌对照，采用聚合酶链反应-限制性片段长度多态性分析。鼻咽癌患者与对照组之间rs712多态性的基因型和等位基因频率无显著差异（GT与GG相比，OR 0.83，95%CI 0.57-1.21；TT与GG相比，OR 1.27，95%CI 0.58-2.75）。我们的数据表明，let-7 miRNA结合位点的KRAS rs712多态性与鼻咽癌风险无关。需要进一步进行更大样本量或其他多态性位点的实验来验证该结果，尤其是在不同种族群体中。
