Pan Xin-Min, Sun Rui-Fen, Li Zhao-Hui, Guo Xiao-Min, Zhang Zhen, Qin Hao-Jie, Xu Guo-Hui, Gao Lin-Bo
Department of Forensic Pathology, College of Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, 471003, People's Republic of China,
Tumour Biol. 2014 Jan;35(1):831-5. doi: 10.1007/s13277-013-1114-3. Epub 2013 Aug 24.
Growing evidence has indicated that polymorphism present in the miRNA binding site of target gene can alter the ability of miRNAs to bind its target gene and modulate the development and progression of cancer. We aimed to investigate the association between let-7 KRAS rs712 polymorphism and the risk of colorectal cancer (CRC). The let-7 KRAS rs712 was analyzed in a case-control study, including 339 CRC patients and 313 age- and sex-matched controls; the relationship between the polymorphism and the clinicopathological features of CRC was also examined. Individuals carrying the let-7 KRAS rs712 TT genotype and T allele had an increased risk of developing CRC (TT vs. GG, adjusted OR = 2.18; 95% CI, 1.00-4.77; T vs. G, adjusted OR = 1.50; 95% CI, 1.15-1.96). Stratified analyses revealed that CRC patients with the let-7 KRAS rs712 TT genotype were more likely to have clinical stage III or IV disease (OR = 3.29, 95% CI, 1.32-8.20) and distant metastasis (OR = 4.70, 95% CI, 1.81-12.25). These findings provide evidence that the let-7 KRAS rs712 polymorphism may play crucial roles in the etiology of CRC.
越来越多的证据表明，靶基因miRNA结合位点存在的多态性可改变miRNA与其靶基因结合的能力，并调节癌症的发生和发展。我们旨在研究let-7 KRAS rs712多态性与结直肠癌（CRC）风险之间的关联。在一项病例对照研究中分析了let-7 KRAS rs712，该研究包括339例CRC患者和313例年龄和性别匹配的对照；还检查了该多态性与CRC临床病理特征之间的关系。携带let-7 KRAS rs712 TT基因型和T等位基因的个体患CRC的风险增加（TT与GG相比，调整后的OR = 2.18；95% CI，1.00 - 4.77；T与G相比，调整后的OR = 1.50；95% CI，1.15 - 1.96）。分层分析显示，具有let-7 KRAS rs712 TT基因型的CRC患者更有可能患有临床III期或IV期疾病（OR = 3.29，95% CI，1.32 - 8.20）和远处转移（OR = 4.70，95% CI，1.81 - 12.25）。这些发现提供了证据，表明let-7 KRAS rs712多态性可能在CRC的病因学中起关键作用。
