Wang Ruihua, Xie Jianguo, Chen Hua, Ren Tingting, Zhang Yicui
Department of Gastroenterology, Shanghai Sixth People's Hospital South Campus, Shanghai Jiao Tong University, Shanghai 201499, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Aug;33(8):1236-9.
To investigate the association of the expressions of angiopoietin-2 (Ang-2), vascular endothelial growth factor (VEGF) in colorectal cancer tissues with Dukes' clinicopathological features.
Ang-2 and Tie-2 mRNA expressions were detected in colorectal cancer tissues, adjacent tissues, and normal tissues by real time-PCR. Quantikine immunoassays were used to measure the protein expressions of Ang-2 and VEGF in the tissues and serum samples.
Ang-2 and Tie-2 levels were significantly higher in the serum of the patients than in the normal tissues (P<0.05), and their expressions were strongly correlated (r=0.879, P=0.000). Tumor tissue Ang-2 and VEGF levels were significantly higher than their levels in the adjacent and normal tissues (P<0.05). In colorectal cancer patients, the peripheral blood level of Ang-2 was significantly higher than that in healthy control subjects, and comparable with that in mesenteric blood (P>0.05). In Dukes' stage C and D patients, serum Ang-2 and VEGF levels were significantly higher than those in patients in Dukes' stage A and B (P<0.05).
Ang-2 and VEGF over expressions may play an important role in the occurrence and progression of colorectal cancer.
探讨结直肠癌组织中血管生成素-2（Ang-2）、血管内皮生长因子（VEGF）的表达与Dukes临床病理特征的关系。
采用实时荧光定量PCR检测结直肠癌组织、癌旁组织及正常组织中Ang-2和Tie-2 mRNA的表达。采用Quantikine免疫分析法检测组织及血清样本中Ang-2和VEGF的蛋白表达。
患者血清中Ang-2和Tie-2水平显著高于正常组织（P<0.05），且二者表达呈强相关（r=0.879，P=0.000）。肿瘤组织中Ang-2和VEGF水平显著高于癌旁及正常组织（P<0.05）。结直肠癌患者外周血Ang-2水平显著高于健康对照者，与肠系膜血中水平相当（P>0.05）。Dukes分期C期和D期患者血清Ang-2和VEGF水平显著高于Dukes分期A期和B期患者（P<0.05）。
Ang-2和VEGF的过表达可能在结直肠癌的发生发展中起重要作用。
