Liu Xinlan, Li Yigong, Wei Jianmin, Zhao Yanjiao
Department of Oncology, General Hospital of Ningxia Medical University, Yinchuan, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Nov;32(11):1658-62.
To study the expression of angiotensin-2 (Ang-2), Tie-2 and vascular endothelial growth factor receptor-2 (VEGFR-2) in colorectal cancer and analyze their relationship with the occurrence, recurrence, metastasis, angiogenesis and prognosis of colorectal cancer.
Immunohistochemistry with SP method was used to detect the expressions of Ang-2, Tie-2 and VEGFR-2 in 118 colorectal cancer, 40 adjacent normal tissue and 40 benign colorectal lesion specimens.
The positivity rates of Ang-2, Tie-2 and VEGFR-2 in colorectal cancer tissue were 74.58%, 69.49%, and 61.02%, respectively, significantly higher than those in the adjacent normal tissues (25.00%, 17.50%, and 17.50%, P<0.05) and benign colorectal lesion tissues (35.00%, 32.50%, and 32.50%, P<0.05). The rates of two or three coexpression were significantly higher than that of a single expression in the cancer tissues (61.02% vs 15.25%). The microvascular density (MVD) of colorectal cancer tissues was 31.43∓10.50, significantly higher than that of the adjacent normal tissues (10.61∓3.76) and benign colorectal lesions (16.89∓3.83) (P<0.05). The expressions of Ang-2, Tie-2, and VEGFR-2 were positively correlated with carcinoembryonic antigen (CEA) and MVD (P<0.05). The expression of Ang-2, but not Tie-2 and VEGFR-2, was positively correlated with CA199. Ang-2, Tie-2, and VEGFR-2 expressions showed significant differences between cases with tumor recurrence/metastasis and those without 5 years after radical mastectomy, and were all positively correlated with the 5-year survival rates (P<0.05).
Ang-2, Tie-2 and VEGFR-2 are involved in the development, invasion, metastasis, and prognosis of colorectal cancer, and play important roles in the angiogenesis of the tumors.
研究血管紧张素 -2（Ang-2）、Tie-2 和血管内皮生长因子受体 -2（VEGFR-2）在结直肠癌中的表达情况，并分析它们与结直肠癌的发生、复发、转移、血管生成及预后的关系。
采用免疫组织化学 SP 法检测 118 例结直肠癌、40 例癌旁正常组织及 40 例大肠良性病变标本中 Ang-2、Tie-2 和 VEGFR-2 的表达。
结直肠癌组织中 Ang-2、Tie-2 和 VEGFR-2 的阳性率分别为 74.58%、69.49%和 61.02%，显著高于癌旁正常组织（25.00%、17.50%和 17.50%，P<0.05）及大肠良性病变组织（35.00%、32.50%和 32.50%，P<0.05）。癌组织中双表达或三表达的比例显著高于单表达比例（61.02%对 15.25%）。结直肠癌组织的微血管密度（MVD）为 31.43±10.50，显著高于癌旁正常组织（10.61±3.76）及大肠良性病变组织（16.89±3.83）（P<0.05）。Ang-2、Tie-2 和 VEGFR-2 的表达与癌胚抗原（CEA）及 MVD 呈正相关（P<0.05）。Ang-2 的表达与 CA199 呈正相关，而 Tie-2 和 VEGFR-2 与 CA199 无相关性。根治性切除术后 5 年，有肿瘤复发/转移的病例与无复发/转移的病例相比，Ang-2、Tie-2 和 VEGFR-2 的表达存在显著差异，且三者均与 5 年生存率呈正相关（P<0.05）。
Ang-2、Tie-2 和 VEGFR-2 参与了结直肠癌的发生、侵袭、转移及预后过程，并在肿瘤血管生成中发挥重要作用。
