Department of Clinical and Molecular Sciences, Università Politecnica delle Marche, Ancona, Italy.
J Biomed Mater Res B Appl Biomater. 2014 Feb;102(2):303-10. doi: 10.1002/jbm.b.33008. Epub 2013 Aug 30.
Coating of orthopaedic or dental Titanium (Ti) implants with extracellular bone matrix components (e.g., Type I collagen or hydroxyapatite) is usually performed to enhance their osseointegration. Aim of the present research is the evaluation of an innovative bone-derived Ti coating, containing bone apatite and Type I bone collagen preserved, in an experimental model. Coated and uncoated titanium implants were inserted into the extra-articular bone of the distal femur of twelve New Zealand White Rabbits. Labelling of bone formation was performed by sequential intraperitoneal administration of three stains. After 45 and 90 days animals were sacrificed. Bone specimens were embedded in a glycol methacrylate resin and sectioned along a plane parallel to the long axis of the implants for histomorphometric, scanning electron microscopy and energy dispersive X-ray analyses. Bone implant contact (BIC), trabecular thickness (Tb.Th) and calcium-phosphorus ratio were measured. Data were subjected to nonparametric Wilcoxon rank-sum test and Student's t test. All implants healed without adverse reactions. After 45 days from implant, significant (p < 0.05) differences in BIC (55.6 ± 17.1% vs. 29.2 ± 20.1%) and Tb.Th (108.7 ± 67.1 µm vs. 66.6 ± 48.6 µm) were observed between coated and uncoated implants. Significant (p < 0.05) differences in BIC (61.3 ± 2.1% vs. 35.7 ± 16.4%) and Tb.Th (211.4 ± 80.8 µm vs. 150.9 ± 61.5 µm) between coated and uncoated implants were also detected after 90 days. No differences were measured in calcium-phosphorous ratio. Our data indicate that Ti integration can be enhanced by the proposed surface coating. This could accelerate stable implant fixation and early or immediate loading of the device.
将细胞外骨基质成分（如 I 型胶原蛋白或羟基磷灰石）涂覆在骨科或牙科钛（Ti）植入物上通常是为了增强其骨整合。本研究的目的是评估一种创新的骨衍生 Ti 涂层，该涂层含有保留的骨磷灰石和 I 型骨胶原蛋白。将涂覆和未涂覆的钛植入物插入 12 只新西兰白兔关节外骨的远端股骨。通过序贯腹腔内注射三种染料来标记骨形成。45 和 90 天后，处死动物。将骨标本嵌入乙二醇甲基丙烯酸酯树脂中，并沿与植入物长轴平行的平面切割，进行组织形态计量学、扫描电子显微镜和能量色散 X 射线分析。测量骨植入物接触（BIC）、小梁厚度（Tb.Th）和钙磷比。数据采用非参数 Wilcoxon 秩和检验和 Student t 检验。所有植入物均无不良反应愈合。植入后 45 天，涂覆和未涂覆植入物之间 BIC（55.6 ± 17.1%比 29.2 ± 20.1%）和 Tb.Th（108.7 ± 67.1 µm 比 66.6 ± 48.6 µm）差异有统计学意义（p < 0.05）。植入后 90 天，涂覆和未涂覆植入物之间 BIC（61.3 ± 2.1%比 35.7 ± 16.4%）和 Tb.Th（211.4 ± 80.8 µm 比 150.9 ± 61.5 µm）差异也有统计学意义（p < 0.05）。钙磷比无差异。我们的数据表明，Ti 整合可以通过提出的表面涂层来增强。这可以加速稳定的植入物固定和设备的早期或立即加载。
