Jayesh P, Seena Jose, Singh I S Bright
National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Lakeside Campus, Fine Arts Avenue, Cochin, 682016 India.
Indian J Virol. 2012 Sep;23(2):244-51. doi: 10.1007/s13337-012-0089-9. Epub 2012 Aug 14.
Development of continuous shrimp cell lines for effective investigation on shrimp viruses remains elusive with an arduous history of over 25 years. Despite presenting challenges to researchers in developing a cell line, the billion dollar aquaculture industry is under viral threat. Advances in molecular biology and various gene transfer technologies for immortalization of cells have resulted in the development of hundreds of cell lines from insects and mammals, but yet not a single cell line has been developed from shrimp and other marine invertebrates. Though improved growth and longevity of shrimp cells in vitro could be achieved by using modified growth media this did not make any leap to spontaneous transformation; probably due to the fact that shrimp cells inhibited neoplastic transformations. Oncogenic induction and immortalization are considered as the possible ways, and an exclusive medium for shrimp cell culture and an appropriate mode of transformation are crucial. In this review status of shrimp cell line development and its future orientation are discussed.
开发用于有效研究虾病毒的连续虾细胞系一直难以实现，有着超过25年的艰巨历程。尽管在开发细胞系方面给研究人员带来了挑战，但价值数十亿美元的水产养殖业却受到病毒威胁。分子生物学和各种用于细胞永生化的基因转移技术的进展已促成了数百种昆虫和哺乳动物细胞系的开发，但尚未从虾和其他海洋无脊椎动物中开发出单一细胞系。虽然通过使用改良的生长培养基可以在体外提高虾细胞的生长和寿命，但这并未使细胞实现自发转化；这可能是因为虾细胞抑制了肿瘤转化。致癌诱导和永生化被认为是可能的途径，而用于虾细胞培养的专用培养基和合适的转化方式至关重要。本文综述了虾细胞系的发展现状及其未来发展方向。
