National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi 682016, India.
Mar Environ Res. 2011 Apr;71(3):169-77. doi: 10.1016/j.marenvres.2010.12.008. Epub 2011 Jan 11.
Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from Penaeus monodon was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos. Using MTT assay 12 h IC(50) values calculated were 31.09 ± 16.27 μM and 5.52 ± 1.16 μM for cadmium chloride and mercuric chloride and 59.94 ± 52.30 mg l(-1) and 186.76 ± 77.00 mg l(-1) for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants.
虾细胞系的缺乏阻碍了对污染物的研究，这些污染物会对虾的健康及其出口价值产生不利影响。在这种情况下，从斑节对虾中开发的原代血淋巴细胞培养物被用于评估两种重金属化合物（氯化镉和氯化汞）和两种有机磷杀虫剂（马拉硫磷和久效磷）的细胞毒性和遗传毒性。使用 MTT 测定法，计算出的 12 h IC（50）值分别为 31.09±16.27 μM 和 5.52±1.16 μM 用于氯化镉和氯化汞，以及 59.94±52.30 mg l（-1）和 186.76±77.00 mg l（-1）用于马拉硫磷和久效磷。通过彗星试验评估这些污染物对血淋巴细胞造成的 DNA 损伤，发现这些污染物使超过 60%的细胞发生 DNA 损伤。该研究表明，血淋巴细胞培养物可作为一种工具，用于量化水产养殖药物、管理化学品和污染物的细胞毒性和遗传毒性。
