Department of Biochemistry and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirão Preto, SP, Brazil.
Toxicon. 2013 Dec 15;76:1-10. doi: 10.1016/j.toxicon.2013.08.063. Epub 2013 Aug 30.
In various types of snake venom, the major toxic components are proteinases and members of the phospholipase A2 family, although other enzymes also contribute to the toxicity. In this study, we evaluated the proteolytic, phospholipase, and L-Amino acid oxidase activities in the venom of five Bothrops species-Bothrops jararaca, Bothrops jararacussu, Bothrops moojeni, Bothrops neuwiedi, and Bothrops alternatus-all of which are used in the production of commercial antivenom, prepared in horses. The enzymatic activities of each species' venom were classified as high, moderate, or low. B. moojeni venom demonstrated the highest enzymatic activity profile, followed by the venom of B. neuwiedi, B. jararacussu, B. jararaca, and B. alternatus. To our knowledge, this is the first study to compare all of these enzymes from multiple species, which is significant in view of the activity of L-amino acid oxidase across Bothrops species.
在各种类型的蛇毒中，主要的毒性成分是蛋白酶和磷脂酶 A2 家族的成员，尽管其他酶也对毒性有贡献。在这项研究中，我们评估了来自五种矛头蝮属蛇的毒液的蛋白水解酶、磷脂酶和 L-氨基酸氧化酶活性 - 矛头蝮属、矛头蝮属、矛头蝮属、矛头蝮属和矛头蝮属，所有这些蛇毒都用于马制备的商业抗蛇毒血清的生产。每种蛇毒的酶活性被归类为高、中或低。B. moojeni 毒液表现出最高的酶活性谱，其次是 B. neuwiedi、B. jararacussu、B. jararaca 和 B. alternatus 毒液。据我们所知，这是首次比较来自多个物种的所有这些酶的研究，鉴于 L-氨基酸氧化酶在整个矛头蝮属物种中的活性，这具有重要意义。
