Cancer Research Unit, Universidad Central del Caribe, School of Medicine, Bayamon - Puerto Rico.
Int J Biol Markers. 2013 Dec 17;28(4):e393-7. doi: 10.5301/JBM.5000043.
The frequency of K-RAS mutations ranges between 30% and 48% among the Caucasian, Asian, and European populations and these mutations are predictors of response to EGFR therapies. We sought to determine the expression of K-RAS gene mutations among colorectal cancer patients in PuertoRico. A retrospective study was conducted to determine the expression of mutant K-RAS among colorectal cancer patients in Puerto Rico between April 2009 and January 2011. The mutant expression of K-RAS was found in 39% (n=195) of the Puerto Rican population, and was more common in the age group of 51-69 years (53.8%) and in males (55.4%, p>0.05). Moreover, mutant K-RAS was more commonly found in tumors of the proximal area (43.8%; p=0.03), with distant metastasis (43.3%, p=0.018), with a mucinous histotype (31.7% p>0.05), and in ulcerated tumors (38.8%, p>0.05). K-RAS mutations were observed on codon 12 (87.7%) and codon 13 (12.3%). The most frequent mutation on codon 12 was 12 ASP (39.5%), followed by 12 VAL (25.4%) that is associated with a significant decrease in overall cancer survival. The mutant expression of K-RAS in cases of rectum carcinoma was 39.5%, where the most common mutation was 12 VAL (37.5%). The frequency of K-RAS mutations in the Puerto Rican population here studied was 39% and mutant K-RAS was associated with advanced colorectal cancer stage, mucinous histotype, and ulcerated tumors.
K-RAS 基因突变频率在白种人、亚洲人和欧洲人群中为 30%至 48%，这些突变是对 EGFR 治疗反应的预测因子。我们试图确定波多黎各的结直肠癌患者中 K-RAS 基因突变的表达情况。进行了一项回顾性研究，以确定 2009 年 4 月至 2011 年 1 月期间波多黎各的结直肠癌患者中 K-RAS 基因突变的表达情况。在波多黎各人群中发现突变型 K-RAS 的表达占 39%（n=195），在 51-69 岁年龄组（53.8%）和男性（55.4%，p>0.05）中更为常见。此外，突变型 K-RAS 更常见于近端区域的肿瘤（43.8%；p=0.03）、远处转移（43.3%；p=0.018）、黏液型组织学（31.7% p>0.05）和溃疡性肿瘤（38.8%；p>0.05）。K-RAS 突变发生在密码子 12（87.7%）和密码子 13（12.3%）。在密码子 12 上最常见的突变是 12 ASP（39.5%），其次是 12 VAL（25.4%），这与整体癌症生存率的显著下降有关。直肠癌病例中 K-RAS 的突变表达为 39.5%，其中最常见的突变是 12 VAL（37.5%）。本研究中波多黎各人群的 K-RAS 突变频率为 39%，突变型 K-RAS 与晚期结直肠癌分期、黏液型组织学和溃疡性肿瘤有关。
