Vetuschi C, Ragno G
Dipartimento Farmaco-Chimico, Università, Bari, Italy.
Farmaco. 1990 Jun;45(6 Suppl):757-69. doi: 10.1002/chin.199052359.
This paper described a rapid ultraviolet spectrophotometric assay for simultaneous determination of tetracyclines (TC + ETC) and their anhydroderivatives (ATC + EATC), by using third- or fourth-derivative spectra recorded on samples in ethanol or dioxane. The analytical procedures are based on linear relationships between specific peak-trough values and the relative concentrations of tetracycline and of ATC-EATC, in mixtures containing TC, ATC and EATC. The toxic EATC can be estimated about half of the anhydrotetracyclines concentration values. The determination limit of anhydroderivatives is 0.05%. A simple purity test of the antibiotic is also reported. Accuracy, precision and linearity are discussed. The method is particularly applicable to routine analysis of products containing TC, in bulk and in some pharmaceutical formulations.
本文描述了一种快速紫外分光光度法，用于同时测定四环素（TC + ETC）及其脱水衍生物（ATC + EATC），该方法是通过记录乙醇或二氧六环中样品的三阶或四阶导数光谱来实现的。分析程序基于特定峰谷值与含TC、ATC和EATC混合物中四环素及ATC - EATC相对浓度之间的线性关系。有毒的EATC的含量约为脱水四环素浓度值的一半。脱水衍生物的测定限为0.05%。本文还报道了一种简单的抗生素纯度检测方法。讨论了该方法的准确性、精密度和线性。该方法特别适用于含TC产品的批量及某些药物制剂的常规分析。
