a Centre for Contact Lens Research, School of Optometry and Vision Science , University of Waterloo , 200 University Avenue West, Waterloo , ON , N2L 3G1 , Canada .
J Biomater Sci Polym Ed. 2014;25(1):18-31. doi: 10.1080/09205063.2013.830914. Epub 2013 Sep 6.
To evaluate the uptake and release of the antifungal agent natamycin encapsulated within poly(D,L-lactide)-dextran nanoparticles (Dex-b-PLA NPs) from model contact lens (CL) materials.
Six model CL materials (gel 1:poly(hydroxyethyl methacrylate, pHEMA); gel 2:85% pHEMA: 15% [Tris(trimethylsiloxy)silyl]-propyl methacrylate (TRIS); gel 3: 75% pHEMA: 25% TRIS; gel 4: 85% N,N dimethylacrylamide (DMAA): 15% TRIS; gel 5:75% DMAA: 25% TRIS; and gel 6: DMAA) were prepared using a photoinitiation procedure. The gels were incubated in: (1) natamycin dissolved in deionized (DI) water and (2) natamycin encapsulated within Dex-b-PLA NPs in dimethylsulfoxide/DI water. Natamycin release from these materials was monitored using UV-visible spectrophotometry at 304 nm over 7 d.
Natamycin uptake by all model CL materials increased between 1 and 7 d (p < 0.001). The uptake of natamycin-NPs was higher than the uptake of the drug alone in DI water (p < 0.05). Drug release was higher in materials containing DMAA than pHEMA (p < 0.05). All gels loaded with natamycin-NPs also released more drug compared to gels soaked with natamycin in DI water (p < 0.001). After 1 h, CL materials loaded with natamycin alone released 28-82% of the total drug release. With the exception of gel 6, this burst released was reduced to 21-54% for CL materials loaded with natamycin-NPs.
Model CL materials loaded with natamycin-Dex-b-PLA NPs were able to release natamycin for up to 12 h under infinite sink conditions. DMAA-TRIS materials may be more suitable for drug delivery of natamycin due to the higher drug release observed with these materials.
评估聚（D，L-丙交酯）-葡聚糖纳米粒子（Dex-b-PLA NPs）包封的抗真菌剂那他霉素在模型隐形眼镜（CL）材料中的摄取和释放。
使用光引发程序制备六种模型 CL 材料（凝胶 1：聚（羟乙基甲基丙烯酸酯），pHEMA；凝胶 2：85%pHEMA：15%[三（三甲基硅氧基）硅基]丙基甲基丙烯酸酯（TRIS）；凝胶 3：75%pHEMA：25%TRIS；凝胶 4：85%N，N-二甲基丙烯酰胺（DMAA）：15%TRIS；凝胶 5：75%DMAA：25%TRIS；凝胶 6：DMAA）。将凝胶孵育在：（1）溶解在去离子（DI）水中的那他霉素和（2）二甲基亚砜/DI 水中包封的 Dex-b-PLA NPs 中的那他霉素。在 7 天内使用紫外可见分光光度法在 304nm 处监测这些材料中那他霉素的释放。
所有模型 CL 材料的那他霉素摄取在 1 至 7 天内增加（p<0.001）。在 DI 水中，那他霉素-NPs 的摄取高于那他霉素单独摄取（p<0.05）。含 DMAA 的材料的药物释放高于含 pHEMA 的材料（p<0.05）。与在 DI 水中浸泡那他霉素的凝胶相比，负载那他霉素-NPs 的所有凝胶也释放了更多的药物（p<0.001）。在 1 小时时，单独负载那他霉素的隐形眼镜材料释放了总药物释放量的 28-82%。除了凝胶 6 之外，负载那他霉素-NPs 的隐形眼镜材料的这种突释被降低至 21-54%。
在无限水槽条件下，负载那他霉素-Dex-b-PLA NPs 的模型 CL 材料能够释放那他霉素长达 12 小时。由于观察到这些材料中药物释放更高，DMAA-TRIS 材料可能更适合那他霉素的药物输送。
