Zhang Jin, Zhao Fei-Fei, Bai Shuang, Zheng Jie, Zheng Ping, Dai Gui-Dong
School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China.
Zhongguo Zhong Yao Za Zhi. 2013 Jun;38(11):1778-82.
To investigate the inhibitory effect of lycium pigment on lipopolysaccharide (LPS)-induced uveitis in rats and its mechanism.
The rat uveitis model was established by 30-day oral administration of lycium pigment (50, 100, 200 mg x kg(-1)) and footpad injection of LPS. Ocular tissues were collected for a histopathological inspection. The protein, nitric oxide and ADMA in aqueous humor, level of inducible nitric oxide synthase (iNOS) in retina, activities of serum total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and content of malondialdehyde (MDA) were determined by using Western blot, ELISA and biochemical methods.
According to the pathological study, lycium pigment (50, 100, 200 mg x kg(-1)) could notably reduce the inflammatory cell infiltration around corpus ciliare matrix of uveitis rats, and the concentration of protein and nitric oxide, and increased ADMA in aqueous humor. Lycium pigment (100, 200 mg x kg(-1)) could significantly inhibit the expression of iNOS in ocular tissues. In addition, lycium pigment (100, 200 mg x kg(-1)) also decrease the activities of serum T-AOC, SOD, GSH-PX, and the content of lipid peroxide MDA.
Lycium pigment has the inhibitory effect on LPS-induced uveitis in rats. Its mechanism is related to the regulation of nitric oxide/ADMA pathway and the improvement of oxidation resistance.
探讨枸杞色素对大鼠脂多糖（LPS）诱导性葡萄膜炎的抑制作用及其机制。
通过连续30天口服枸杞色素（50、100、200mg·kg⁻¹）并足底注射LPS建立大鼠葡萄膜炎模型。收集眼组织进行组织病理学检查。采用蛋白质免疫印迹法、酶联免疫吸附测定法及生化方法检测房水中蛋白质、一氧化氮和不对称二甲基精氨酸（ADMA）含量、视网膜中诱导型一氧化氮合酶（iNOS）水平、血清总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）活性以及丙二醛（MDA）含量。
病理研究显示，枸杞色素（50、100、200mg·kg⁻¹）可显著减轻葡萄膜炎大鼠睫状体基质周围的炎性细胞浸润，降低房水中蛋白质和一氧化氮浓度，并增加ADMA含量。枸杞色素（100、200mg·kg⁻¹）可显著抑制眼组织中iNOS的表达。此外，枸杞色素（100、200mg·kg⁻¹）还可降低血清T-AOC、SOD、GSH-PX活性以及脂质过氧化物MDA含量。
枸杞色素对大鼠LPS诱导性葡萄膜炎具有抑制作用。其机制与调节一氧化氮/ADMA通路及提高抗氧化能力有关。
