Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China.
J Agric Food Chem. 2010 Apr 28;58(8):4731-6. doi: 10.1021/jf904572a.
Endotoxin-induced uveitis (EIU), a useful animal model of ocular inflammation, is induced by injection of lipopolysacharide (LPS). These experiments showed that the nitric oxide (NO) level significantly increased in the whole eye homogenate of BALB/C mice 24 h after footpad injection of LPS at a dosage of 100 mg/mouse. However, the elevated NO level was significantly reduced by oral administration of bilberry extract (containing 42.04% anthocyanins) at dosages of 50, 100, and 200 mg/kg/day for 5 days before the LPS injection. In addition, bilberry extract decreased malondialdehyde (MDA) level and increased oxygen radical absorbance capacity (ORAC) level, glutathione (GSH) level, vitamin C level, and total superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. Moreover, bilberry extract increased expression of copper/zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD), and GPx mRNA. Taken together, bilberry extract showed protective effects against EIU, whereas the effects of bilberry extract (100 and 200 mg/kg/day, 5 days) were dose-dependent. In conclusion, these results provide new evidence to elucidate the beneficial effects of bilberry extract on eye health.
内毒素性葡萄膜炎（EIU）是一种有用的眼部炎症动物模型，通过注射脂多糖（LPS）诱导。这些实验表明，在给 BALB/C 小鼠足底注射 100mg/mouse LPS 后 24 小时，整个眼球匀浆中的一氧化氮（NO）水平显著升高。然而，通过在 LPS 注射前 5 天每天口服蓝莓提取物（含有 42.04%花青素）50、100 和 200mg/kg，NO 水平的升高显著降低。此外，蓝莓提取物降低了丙二醛（MDA）水平，增加了氧自由基吸收能力（ORAC）水平、谷胱甘肽（GSH）水平、维生素 C 水平以及总超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性。此外，蓝莓提取物增加了铜/锌超氧化物歧化酶（CuZnSOD）、锰超氧化物歧化酶（MnSOD）和 GPx mRNA 的表达。总之，蓝莓提取物对 EIU 表现出保护作用，而蓝莓提取物（100 和 200mg/kg/天，5 天）的作用呈剂量依赖性。总之，这些结果为阐明蓝莓提取物对眼睛健康的有益作用提供了新的证据。
