Nano Medical Engineering Laboratory, RIKEN , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
J Am Chem Soc. 2013 Sep 18;135(37):13632-5. doi: 10.1021/ja406724k. Epub 2013 Sep 6.
A pre-type sensitizer for a lanthanide complex on an oligonucleotide was successfully converted to a perfect final structure in a target DNA/RNA-templated reaction, without any chemical reagent or enzyme, under neutral conditions. The final form of the lanthanide-oligonucleotide provided a long-lived luminescence signal, appropriate for time-gated luminescence analysis and signal amplification. Target DNA/RNA-assisted time-gated luminescence analysis is a powerful tool for elimination of autofluorescence and detection of target RNA in living bacterial cells.
在中性条件下，无需任何化学试剂或酶，寡核苷酸上镧系元素配合物的预型敏化剂在目标 DNA/RNA 模板反应中成功转化为完美的最终结构。镧系元素-寡核苷酸的最终形式提供了长寿命的发光信号，适用于时间门控发光分析和信号放大。目标 DNA/RNA 辅助的时间门控发光分析是消除自发荧光和检测活细菌细胞中目标 RNA 的强大工具。
