基于重复DNA序列的模板导向连接：一种探测亨廷顿氏病DNA长度的化学方法
Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA.
作者信息
Kern Anika, Seitz Oliver
机构信息
Institut für Chemie , Humboldt-Universität zu Berlin , Brook-Taylor-Straße 2 , 12489 Berlin , Germany . Email:
出版信息
Chem Sci. 2015 Jan 1;6(1):724-728. doi: 10.1039/c4sc01974a. Epub 2014 Sep 16.
Abstract
Several genomic disorders are caused by an excessive number of DNA triplet repeats. We developed a DNA-templated reaction in which product formation occurs only when the number of repeats exceeds a threshold indicative for the outbreak of Chorea Huntington. The combined use of native chemical PNA ligation and auxiliary DNA probes enabled reactions on templates obtained from human genomic DNA.
摘要
几种基因组疾病是由过多的DNA三联体重复序列引起的。我们开发了一种DNA模板反应，其中只有当重复序列的数量超过指示亨廷顿舞蹈症发作的阈值时才会发生产物形成。天然化学肽核酸连接和辅助DNA探针的联合使用使得能够在从人类基因组DNA获得的模板上进行反应。
Zotero 插件