马来西亚蛇类中 Sarcocystis spp. 的遗传组合。
Genetic assemblage of Sarcocystis spp. in Malaysian snakes.
机构信息
Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
出版信息
Parasit Vectors. 2013 Sep 9;6(1):257. doi: 10.1186/1756-3305-6-257.
BACKGROUND
Sarcocystis species are protozoan parasites with a wide host range including snakes. Although there were several reports of Sarcocytis species in snakes, their distribution and prevalence are still not fully explored.
METHODS
In this study, fecal specimens of several snake species in Malaysia were examined for the presence of Sarcocystis by PCR of 18S rDNA sequence. Microscopy examination of the fecal specimens for sporocysts was not carried as it was difficult to determine the species of the infecting Sarcocystis.
RESULTS
Of the 28 snake fecal specimens, 7 were positive by PCR. BLASTn and phylogenetic analyses of the amplified 18S rDNA sequences revealed the snakes were infected with either S. nesbitti, S. singaporensis, S. zuoi or undefined Sarcocystis species.
CONCLUSION
This study is the first to report Sarcocystis infection in a cobra, and S. nesbitti in a reticulated python.
背景
肌肉孢子虫属是一种具有广泛宿主范围的原生动物寄生虫，包括蛇类。尽管有几篇关于蛇类肌肉孢子虫属的报道，但它们的分布和流行情况仍未得到充分探索。
方法
在这项研究中，通过聚合酶链反应（PCR）对 18S rDNA 序列检测了来自马来西亚的几种蛇的粪便样本中是否存在肌肉孢子虫属。由于难以确定感染肌肉孢子虫属的种类，因此未对粪便样本进行显微镜检查以寻找孢子囊。
结果
28 份蛇类粪便样本中，有 7 份通过 PCR 呈阳性。扩增的 18S rDNA 序列的 BLASTn 和系统发育分析显示，这些蛇感染了 nesbitti 肌肉孢子虫、singaporensis 肌肉孢子虫、zuoi 肌肉孢子虫或未定义的肌肉孢子虫属。
结论
本研究首次报道了眼镜蛇感染肌肉孢子虫属，以及网纹蟒感染 nesbitti 肌肉孢子虫属。
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