Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan.
Influenza Other Respir Viruses. 2013 Nov;7(6):1210-7. doi: 10.1111/irv.12150. Epub 2013 Sep 6.
Allergic asthma is a globally respiratory inflammatory disease. Influenza virus is a respiratory pathogen that causes yearly epidemics and results in high rates of morbidity and mortality. Patients with allergic asthma had a more severe symptom and a higher mortality when they were infected with influenza virus. Hence, influenza vaccination is recommended for patients with asthma.
We evaluated the efficacy and effects of influenza vaccination on allergic asthma in a mouse model.
Ovalbumin-immunized mice were inoculated with inactivated influenza virus A/Puerto Rico/8/34 (PR8) as vaccines and morbidity or mortality and allergic asthma features of these mice were analyzed.
Mice inoculated with inactivated PR8 induced high levels of anti-PR8 IgG2a and upregulation of Toll-like receptor (TLR) 7. Vaccinated allergic mice were healthy when they were challenged with live influenza virus while none of non-vaccinated allergic mice survived. Furthermore, inactivated influenza virus vaccine induced neither extra airway inflammation nor asthma features such as IgE, airway hyper-reactivity, and eosinophilia in allergic mice. Particularly, decreased frequency of immune cell infiltrated airways and Th2 cytokines IL-4 and IL-6 production in the bronchoalveolar lavage fluid were noted in vaccinated allergic mice. These results suggested that inactivated influenza virus vaccine is efficient to protect allergic mice from further influenza infection, and it does not exacerbate but reduces IL-4 and IL-6 of allergic asthma.
Influenza vaccination is essential and efficient for allergic subjects to protect influenza virus infection.
过敏性哮喘是一种全球性呼吸道炎症性疾病。流感病毒是一种呼吸道病原体，每年都会引发流行，导致高发病率和死亡率。患有过敏性哮喘的患者在感染流感病毒时症状更严重，死亡率更高。因此，建议哮喘患者接种流感疫苗。
我们在小鼠模型中评估了流感疫苗对过敏性哮喘的疗效和影响。
用卵清蛋白免疫的小鼠接种灭活的流感病毒 A/Puerto Rico/8/34（PR8）作为疫苗，并分析这些小鼠的发病率或死亡率和过敏性哮喘特征。
接种灭活 PR8 的小鼠诱导高水平的抗 PR8 IgG2a 和 Toll 样受体（TLR）7 的上调。接种流感疫苗的过敏性小鼠在受到活流感病毒攻击时保持健康，而未接种疫苗的过敏性小鼠无一存活。此外，灭活流感病毒疫苗在过敏性小鼠中既没有引起额外的气道炎症，也没有引起哮喘特征，如 IgE、气道高反应性和嗜酸性粒细胞增多。特别是，在接种疫苗的过敏性小鼠的支气管肺泡灌洗液中，免疫细胞浸润气道的频率和 Th2 细胞因子 IL-4 和 IL-6 的产生减少。这些结果表明，灭活流感病毒疫苗能有效保护过敏性小鼠免受流感感染，且不会加重而是减轻过敏性哮喘的 IL-4 和 IL-6。
流感疫苗对于过敏性患者预防流感病毒感染至关重要且有效。
