Xie Chang-Hao, Wang Yuan-Yuan, Li Zhi-Jun, Tang Jie, Li Bai-Qing
Department of Rheumatology and Immunology, the First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu 233004, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Apr;28(4):429-32.
To detect expression of (programmed death ligand 1, PD-L1)on CD14(+) Monocyte(Mo)in the patients with systemic lupus erythematosus(SLE)and their clinical significance is analyzed.
The expression of PD-L1 on the CD14(+) Mo was examined in patients with 51 active SLE and 38 healthy controls(HC) by flow cytometry. The proportions of expression of PD-L1 on CD14(+) Mo were compared between not only inactive or active SLE patients and healthy controls(HC), but also between patients with lupus nephritis and without lupus nephritis. Correlation with clinical manifestations and laboratory findings was analyzed.
The Proportions of CD14(+) PD-L1(+) Mo were significantly increased in active and inactive SLE patients as compared with HC(all P<0.05). The proportions of CD14(+) PD-L1(+) Mo in SLE patients with nephritis were significantly higher than those in patients without nephritis. A positive correlation was observed for proportions of CD14(+) PD-L1(+) Mo with SLEDAI score, and amounts of proteinuria. Proportions of CD14(+) PD-L1(+) Mo in SLE patients with anti-dsDNA, anti-Sm, anti-U1RNP and anti-nucleosome were higher than those in SLE patients without those antibody(P<0.05).
The aberrations of proportions of CD14(+) PD-L1(+) Mo were observed in patients with SLE. Proportions of CD14(+) PD-L1(+) Mo were correlated with disease activity and production of antibody.
检测系统性红斑狼疮（SLE）患者CD14（+）单核细胞（Mo）上程序性死亡配体1（PD-L1）的表达，并分析其临床意义。
采用流式细胞术检测51例活动期SLE患者和38例健康对照（HC）中CD14（+）Mo上PD-L1的表达。比较非活动期或活动期SLE患者与健康对照之间，以及狼疮肾炎患者和无狼疮肾炎患者之间CD14（+）Mo上PD-L1表达的比例。分析其与临床表现和实验室检查结果的相关性。
与HC相比，活动期和非活动期SLE患者CD14（+）PD-L1（+）Mo的比例显著增加（所有P<0.05）。肾炎SLE患者CD14（+）PD-L1（+）Mo的比例显著高于无肾炎患者。观察到CD14（+）PD-L1（+）Mo的比例与SLE疾病活动指数（SLEDAI）评分及蛋白尿定量呈正相关。抗双链DNA、抗Sm、抗U1RNP和抗核小体抗体阳性的SLE患者CD14（+）PD-L1（+）Mo的比例高于无这些抗体的SLE患者（P<0.05）。
SLE患者存在CD14（+）PD-L1（+）Mo比例异常。CD14（+）PD-L1（+）Mo的比例与疾病活动及抗体产生相关。
