Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Private Bag 75, Hobart 7001, Australia.
J Chromatogr A. 2013 Oct 11;1311:121-6. doi: 10.1016/j.chroma.2013.08.077. Epub 2013 Aug 27.
The preparation of composite monolithic cryopolymers is presented. These novel porous materials were prepared in capillary format at -70°C using poly(ethyleneglycol) diacrylate (PEGDA) Mw 258 as the single monomer and a mixture of dioxane and water as the porogen. Positively (NR4(+)) or negatively (SO3(-)) charged nanoparticles were incorporated within the polymeric structure by direct addition of their suspensions to the polymerisation mixture. In contrast to our previous report using neutral nanoparticles, the trapping of charged nanoparticles is mostly observed at the polymer surface. The incorporation of these nanostructures improved the chromatographic separations of standard proteins under a hydrophobic interaction chromatography (HIC) separation mode. Moreover, the presence of ionic groups on the polymer surface allowed the application of these columns under ion-exchange (IEX) conditions. The results obtained in this work show that the functionalisation of monolithic columns by direct addition of nanoparticles is a good alternative towards the modification of monolithic polymers without altering the polymeric scaffold.
本文介绍了复合整体式 cryopolymers 的制备。这些新型多孔材料是在 -70°C 下使用聚乙二醇二丙烯酸酯 (PEGDA)Mw258 作为单一单体和二恶烷和水的混合物作为致孔剂在毛细管中制备的。正电荷 (NR4(+)) 或负电荷 (SO3(-)) 纳米颗粒通过将其悬浮液直接加入聚合混合物中而掺入聚合物结构中。与我们之前使用中性纳米颗粒的报告不同，带电荷的纳米颗粒的捕获主要观察到在聚合物表面。这些纳米结构的掺入改善了标准蛋白质在疏水相互作用色谱 (HIC) 分离模式下的色谱分离。此外，聚合物表面上的离子基团允许在离子交换 (IEX) 条件下使用这些柱。本工作得到的结果表明，通过直接添加纳米颗粒对整体式柱进行功能化是在不改变聚合物支架的情况下对整体式聚合物进行改性的一种很好的替代方法。
