Department of Chemistry, National University of Singapore, Singapore 117543.
J Am Chem Soc. 2012 Sep 12;134(36):14678-81. doi: 10.1021/ja306265n. Epub 2012 Aug 27.
A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).
基于连接酶链反应（LCR）产物检测和金纳米粒子（AuNPs）作为信号发生器的简单、超灵敏比色 DNA 测定法已经开发出来。在 LCR 过程中，AuNPs 连接在一起，经过足够多的热循环后，实时产生明显的颜色变化。该方案的累积性质产生了 20 aM 的检测限，选择性因子为 10（3）。
