Horsch A, Köhler K, Ellwart-Tschürz M, Schmid H P
Biologisches Institut, Universität Stuttgart, FRG.
FEBS Lett. 1990 Sep 3;269(2):336-40. doi: 10.1016/0014-5793(90)81188-t.
Viral messengers were used to select and purify prosomes and prosomal RNA from subribosomal fractions of HeLa cells and mouse erythroblasts. Adenovirus mRNA immobilized on oligo(dT)-cellulose and tobacco mosaic virus RNA (TMV) sedimenting in sucrose gradients associated strongly with prosomes at high salt conditions forming intermolecular RNA-RNA hybrids between prosomal RNA and viral RNA. Hybrid selection of small cytoplasmic RNAs with immobilized TMV-RNA revealed a RNA species migrating at the same position as prosomal RNA. The possible existence of a box-like sequence involved in hybridization will be discussed.
利用病毒信使从HeLa细胞和小鼠成红细胞的亚核糖体组分中筛选并纯化前体微粒和前体微粒RNA。固定在寡聚（dT）-纤维素上的腺病毒mRNA以及在蔗糖梯度中沉降的烟草花叶病毒RNA（TMV）在高盐条件下与前体微粒强烈结合，在前体微粒RNA和病毒RNA之间形成分子间RNA-RNA杂交体。用固定化的TMV-RNA对小细胞质RNA进行杂交筛选，发现一种RNA与前体微粒RNA迁移到相同位置。将讨论参与杂交的盒状序列的可能存在情况。
