Bendahan D, Confort-Gouny S, Kozak-Reiss G, Cozzone P J
Centre de Résonance Magnétique Biologique et Médicale, Faculté de Médecine de Marseille, France.
FEBS Lett. 1990 Sep 3;269(2):402-5. doi: 10.1016/0014-5793(90)81203-z.
31P NMR spectroscopy at 4.7 T has been used to follow changes in phosphorylated metabolites and pHi in the flexor digitorum superficialis muscle of 15 healthy volunteers subjected to a rest-exercise-recovery protocol. Phosphomonoesters (Pme) increased during exercise and exhibited a delayed recovery to resting level. During early recovery, Pi fell below resting concentration without correlated PCr oversynthesis while Pme level stayed above its resting value. The sum Pi + Pme remained constant. These observations suggest that Pi could be trapped into the glycogenolytic pathway during exercise leading to Pme production. This trapping and the slow Pme recovery could account for transient Pi disappearance observed during recovery.
利用4.7T的31P核磁共振波谱技术，对15名健康志愿者的指浅屈肌进行静息-运动-恢复实验，以追踪磷酸化代谢产物和细胞内pH值的变化。运动期间磷酸单酯（Pme）增加，并在恢复至静息水平时出现延迟。在早期恢复阶段，无机磷酸盐（Pi）降至静息浓度以下，而磷酸肌酸（PCr）没有过度合成，同时Pme水平保持在静息值之上。Pi + Pme的总和保持不变。这些观察结果表明，运动期间Pi可能被困在糖原分解途径中，导致Pme生成。这种滞留以及Pme的缓慢恢复可能是恢复期间观察到的Pi短暂消失的原因。
