College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Int J Syst Evol Microbiol. 2014 Jan;64(Pt 1):66-71. doi: 10.1099/ijs.0.050955-0. Epub 2013 Sep 6.
A Gram-stain-negative, non-motile and aerobic bacterium, designated CF17(T), was isolated from coastal planktonic seaweeds, East China Sea. The isolate grew at 18-37 °C (optimum 25-28 °C), pH 6.5-9.0 (optimum 7.0-8.0) and with 0-5 % NaCl (optimum 1-2 %, w/v) and 0.5-10 % sea salts (optimum 2-3 %, w/v). Growth of strain CF17(T) could be stimulated prominently by supplementing the growth medium with the autoclaved supernatant of a culture of strain CF5, which was isolated from the same sample along with strain CF17(T). The cell morphology of strain CF17(T) was a bean-shaped rod consisting of a swollen end and a long prostheca. The phylogenetic analysis of 16S rRNA gene sequences indicated that strain CF17(T) clustered with Gemmobacter nectariphilus DSM 15620(T) within the genus Gemmobacter. The DNA G+C content of strain CF17(T) was 61.4 mol%. The respiratory quinone was ubiquinone Q-10. The major fatty acids included C18 : 1ω7c and C18 : 0. The polar lipids of strain CF17(T) consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two uncharacterized phospholipids, one uncharacterized aminolipid, three uncharacterized glycolipids and one uncharacterized lipid. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain CF17(T) ( = CGMCC 1.11024(T) = JCM 18498(T)) is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter megaterium sp. nov. is proposed.
一株革兰氏阴性、非运动和需氧细菌，被命名为 CF17(T)，从东海沿海浮游海藻中分离得到。该分离株在 18-37°C（最佳温度 25-28°C）、pH 6.5-9.0（最佳 pH 7.0-8.0）和 0-5%NaCl（最佳 1-2%，w/v）和 0.5-10%海水盐（最佳 2-3%，w/v）中生长。通过在生长培养基中添加与从同一样品中与 CF17(T)一起分离的 CF5 菌株的灭菌培养物上清液，可以显著刺激 CF17(T)菌株的生长。CF17(T)菌株的细胞形态为豆形棒状，由一个肿胀的末端和一个长的菌毛组成。16S rRNA 基因序列的系统发育分析表明，CF17(T)菌株与 Gemmobacter nectariphilus DSM 15620(T)聚在 Gemmobacter 属内。CF17(T)菌株的 DNA G+C 含量为 61.4 mol%。呼吸醌为泛醌 Q-10。主要脂肪酸包括 C18:1ω7c 和 C18:0。CF17(T)菌株的极性脂由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、两种未鉴定的磷脂、一种未鉴定的胺脂、三种未鉴定的糖脂和一种未鉴定的脂组成。基于表型、系统发育和化学分类学数据，CF17(T)菌株（ = CGMCC 1.11024(T) = JCM 18498(T)）被认为代表 Gemmobacter 属的一个新种，因此提议将其命名为巨 Gemmobacter megaterium sp. nov.。
