Kang Ji Young, Kim Mi-Jung, Chun Jeesun, Son Kyung Pyo, Jahng Kwang Yeop
Industrial Microbiology and Bioprocess Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si, 580-185, Republic of Korea.
Department of Life Sciences, Chonbuk National University, Jeonju-si, 561-756, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5019-5025. doi: 10.1099/ijsem.0.002403. Epub 2017 Oct 12.
A Gram-stain-negative, non-motile and facultative anaerobic bacterium, designated CAM-8, was isolated from an artificial fountain at Chonbuk National University, South Korea. The novel strain grew at 20-37 °C (optimum 25 °C), pH 5.5-7.0 (optimum 6.0) and with 0-2 % NaCl (optimum 0 %). Oxidase and catalase activities were positive. The cell morphology of strain CAM-8 was atypical rods 0.6-0.8 µm in width and 4.5-6.5 µm in length, with a peaked tip and sometimes a bulb shape. CAM-8 existed as single cells, and as pairs or chains of cells. The phylogenetic analysis of 16S rRNA gene sequences indicated that strain CAM-8 clustered with Gemmobacter nectariphilus JCM 11959 and Gemmobactermegaterium JCM 18498 within the genus Gemmobacter. The DNA G+C content of strain CAM-8 was 65.9 mol%. The respiratory quinone was ubiquinone Q-10. The major fatty acids were C18 : 1ω7c and/or C18 : 1ω6c. The polar lipids of strain CAM-8 consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two uncharacterized phospholipids, an uncharacterized aminolipid, an uncharacterized glycolipid, an uncharacterized aminophospholipid and four uncharacterized lipids. On the basis of phenotypic, phylogenetic and chemotaxonomic data, strain CAM-8 (=KACC 19224=JCM 31905) is considered to represent a novel species of the genus Gemmobacter, for which the name Gemmobacter straminiformis sp. nov. is proposed.
从韩国全北国立大学的一个人工喷泉中分离出一株革兰氏阴性、无运动性兼性厌氧细菌，命名为CAM-8。该新菌株在20-37 °C（最适温度25 °C）、pH 5.5-7.0（最适pH 6.0）和0-2 % NaCl（最适0 %）条件下生长。氧化酶和过氧化氢酶活性呈阳性。菌株CAM-8的细胞形态为非典型杆菌，宽度为0.6-0.8 µm，长度为4.5-6.5 µm，顶端尖锐，有时呈球状。CAM-8以单细胞形式存在，也有成对或成链的细胞形式。16S rRNA基因序列的系统发育分析表明，菌株CAM-8与Gemmobacter属中的嗜花蜜芽胞杆菌JCM 11959和巨大芽胞杆菌JCM 18498聚类在一起。菌株CAM-8的DNA G+C含量为 65.9 mol%。呼吸醌为泛醌Q-10。主要脂肪酸为C18 : 1ω7c和/或C18 : 1ω6c。菌株CAM-8的极性脂质由磷脂酰乙醇胺､磷脂酰甘油､磷脂酰胆碱､两种未鉴定的磷脂､一种未鉴定的氨基脂质､一种未鉴定的糖脂､一种未鉴定的氨基磷脂和四种未鉴定的脂质组成。基于表型、系统发育和化学分类学数据，菌株CAM-8（=KACC 19224=JCM 31905）被认为代表芽胞杆菌属的一个新物种，为此提出芽胞杆菌新种Gemmobacter straminiformis的名称。
