College of Pharmacy, South-Central University for Nationalities, Wuhan, 430074, PR China.
Biosens Bioelectron. 2014 Feb 15;52:29-35. doi: 10.1016/j.bios.2013.08.031. Epub 2013 Aug 27.
A "turn off-on" mode has been established by using the interaction between platinum anticancer drugs and DNA as input signal and the fluorescence reversible change of quantum dots (QDs) as output signal. The QDs fluorescence can be quenched by platinum anticancer drugs via photo-induced electron transfer process, rendering the system into "turn off" status, and the system can then be "turned on" when fluorescence is restored due to covalent conjugation between DNA and platinum anticancer drugs. This dual-directional fluorescence change realized the detection of cisplatin and DNA, overcoming the selectivity problem commonly existed in the traditional mono-directional fluorescence detection mode. The reversible fluorescent "turn off-on" mode has been further employed to study the interactions between DNA and different platinum anticancer drugs (cisplatin, oxaliplatin and carboplatin). Furthermore, the impacts of different types of DNAs (different in base sequence, chain length and ssDNA/dsDNA) on the mode are also explored. This simple, fast and convenient spectroscopic method owns promising applications in the study on interaction between medical molecules and DNA, and in biochemical detections.
建立了一种“开-关”模式，将铂类抗癌药物与 DNA 的相互作用作为输入信号，将量子点（QDs）的荧光可逆变化作为输出信号。铂类抗癌药物可通过光诱导电子转移过程猝灭 QDs 荧光，使体系处于“关”状态，当 DNA 与铂类抗癌药物发生共价键合导致荧光恢复时，体系又可“开”。这种双向荧光变化实现了顺铂和 DNA 的检测，克服了传统单向荧光检测模式中普遍存在的选择性问题。该可逆荧光“关-开”模式进一步用于研究 DNA 与不同铂类抗癌药物（顺铂、奥沙利铂和卡铂）的相互作用。此外，还探讨了不同类型 DNA（碱基序列、链长和 ssDNA/dsDNA 不同）对该模式的影响。这种简单、快速、方便的光谱方法有望应用于医学分子与 DNA 相互作用的研究以及生化检测中。
