Gong Yan, Fan Zhefeng
Department of Chemistry, Shanxi Normal University, Linfen, 041004, People's Republic of China.
J Fluoresc. 2016 Mar;26(2):385-93. doi: 10.1007/s10895-015-1699-6. Epub 2015 Dec 14.
A sensitive phosphorescent sensor based on riboflavin (RF)-modulated mercaptopropionic acid (MPA)-capped Mn-doped ZnS quantum dots (QDs) was developed and utilized as room-temperature phosphorescence (RTP) sensor for DNA detection. The RTP of the MPA-capped Mn-doped ZnS QDs was stored via photoinduced electron transfer by RF, and formed an electrochemically nonactive QDs/RF nanohybrids through electrostatic attraction. In the presence of DNA, RF could bind with DNA, which has a double helical structure, via electrostatic interaction and intercalation. RF can be removed from the surface of the QDs, thus releasing the RTP of the QDs. On the basis of this principle, an RTP sensor for DNA detection was developed. Under optimal conditions, the detection limit for DNA was 15 μg mL(-1), the relative standard deviation was 1.9 %, and the method recovery ranged from 97 % to 103 %. The proposed method was applied to biological fluids, in which satisfactory results were obtained.
基于核黄素（RF）调制的巯基丙酸（MPA）包覆的锰掺杂硫化锌量子点（QDs）构建了一种灵敏的磷光传感器，并将其用作室温磷光（RTP）传感器用于DNA检测。MPA包覆的锰掺杂硫化锌量子点的RTP通过RF的光致电子转移得以存储，并通过静电吸引形成了电化学惰性的量子点/核黄素纳米杂化物。在DNA存在的情况下，RF可通过静电相互作用和嵌入作用与具有双螺旋结构的DNA结合。RF可从量子点表面去除，从而释放量子点的RTP。基于这一原理，开发了一种用于DNA检测的RTP传感器。在最佳条件下，DNA的检测限为15 μg mL(-1)，相对标准偏差为1.9%，方法回收率在97%至103%之间。该方法应用于生物流体检测，获得了满意的结果。
