Guilloteau D, Perdrisot R, Calmettes C, Baulieu J L, Lecomte P, Kaphan G, Milhaud G, Besnard J C, Jallet P, Bigorgne J C
Centre Hospitalier Universitaire, Tours, France.
J Clin Endocrinol Metab. 1990 Oct;71(4):1064-7. doi: 10.1210/jcem-71-4-1064.
A new calcitonin (CT) immunoradiometric assay, using anti-11-7 and anti-24-32 CT fragment monoclonal antibodies was evaluated and compared to classical RIA. The sensitivity was 2.5 ng/L, the normal basal level (n = 83) was lower than 10 ng/L, the response to pentagastrin stimulation in control subjects was absent in nine and between 10-30 ng/L in nine others. (mean, 15.4). In patients with renal failure the basal level was increased between 10-52 ng/L. In patients with medullary thyroid carcinoma (MTC; n = 28), the basal level was between 189-28,900 ng/L. A pentagastrin test was performed as screening for familial MTC in eight patients with confirmed MTC at subsequent surgery; the calcitonin peak was equal or greater than 38 ng/L. Large differences exist between CT levels measured by RIA and immunoradiometric assay. The latter method provides a greater sensitivity to pentagastrin test and allows a better identification of microcarcinoma in hereditary cases of MTC.
采用抗 11 - 7 和抗 24 - 32 降钙素（CT）片段单克隆抗体的新型 CT 免疫放射分析方法进行了评估，并与经典放射免疫分析（RIA）进行了比较。其灵敏度为 2.5 ng/L，正常基础水平（n = 83）低于 10 ng/L，对照组中 9 人对五肽胃泌素刺激无反应，另外 9 人反应在 10 - 30 ng/L 之间（平均值为 15.4）。肾衰竭患者的基础水平在 10 - 52 ng/L 之间升高。甲状腺髓样癌（MTC；n = 28）患者的基础水平在 189 - 28,900 ng/L 之间。对 8 例确诊 MTC 且后续行手术治疗的患者进行五肽胃泌素试验以筛查家族性 MTC；降钙素峰值等于或大于 38 ng/L。RIA 法和免疫放射分析法测得的 CT 水平存在较大差异。后一种方法对五肽胃泌素试验具有更高的灵敏度，并且在 MTC 遗传性病例中能更好地识别微癌。
