Visiting Professor, National Institution for Academic Degrees and University Evaluation, Tokyo, Japan .
Proc Jpn Acad Ser B Phys Biol Sci. 2007 May;83(3):77-96. doi: 10.2183/pjab.83.77.
Sexual diversity of ADG in Harderian gland of golden hamster was demonstrated on TLC. Female ADG contained iso- and anteiso-branched acyl and alkyl components, but male ADG contained only straight chain ones, which suggested the hormonal control of the expression of acyl-CoA dehydrogenases in the catabolism of BCAA. Acyl-CoA dehydrogenases were not expressed in the absence of testosterone, and then isovaleryl-CoA, 2-methylbutyryl-CoA, and isobutyryl-CoA accumulated, and acted as primers for the synthesis of iso- and anteiso-branched fatty acids. The incorporation of [U-(14)C] leucine into lipids was monitored by TLC. The cholesterol fraction was labeled in males but not in female, which means that cholesterol was not produced from BCAA in female gland due to the lack of expression of acyl-CoA dehydrogenases. We monitored the behavior of male hamsters toward female gland lipids, and found slightly greater attractiveness in female ones than that in male ones although the difference was not significant. Considering the lifestyle of golden hamster in nature, we propose a hypothesis that the lipids from the Harderian gland of golden hamster serve as a pheromone to declare their territory and to seek the mate with good congeniality.
哈德氏腺 ADG 的性二态性在 TLC 上得到证实。雌性 ADG 含有同型和异型支链酰基和烷基成分，而雄性 ADG 只含有直链成分，这表明 BCAA 分解代谢中酰基辅酶 A 脱氢酶的表达受到激素的控制。在缺乏睾酮的情况下，酰基辅酶 A 脱氢酶不表达，然后异戊酰基辅酶 A、2-甲基丁酰基辅酶 A 和异丁酰基辅酶 A 积累，并作为合成同型和异型支链脂肪酸的引物。通过 TLC 监测 [U-(14)C]亮氨酸掺入脂质的情况。胆固醇馏分在雄性中标记，但在雌性中不标记，这意味着由于酰基辅酶 A 脱氢酶的表达缺失，胆固醇不会从 BCAA 在雌性腺中产生。我们监测了雄性仓鼠对雌性腺脂质的行为，发现尽管差异不显著，但雌性的吸引力略大于雄性。考虑到金黄仓鼠在自然界中的生活方式，我们提出了一个假设，即金黄仓鼠哈德氏腺的脂质作为一种信息素，用于宣告其领地，并寻找具有良好亲缘关系的伴侣。
