拉曼和红外光谱法测定蜂花粉的营养参数。

Determination of nutritional parameters of bee pollen by Raman and infrared spectroscopy.

机构信息

Department of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383, Wrocław, Poland.

Department of Food Storage and Technology, Faculty of Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, 37 Chełmońskiego, 51-630, Wrocław, Poland.

出版信息

Talanta. 2020 May 15;212:120790. doi: 10.1016/j.talanta.2020.120790. Epub 2020 Jan 28.

DOI:10.1016/j.talanta.2020.120790

PMID:32113553

Abstract

Vibrational spectroscopy was applied to determine macronutrient levels, total polyphenols (TPs) content, antioxidant activity, pH and color parameters in bee pollens. Raman, attenuated total reflection and diffuse reflectance spectra in the mid- and near-infrared regions were recorded for the homogenized pollen granules. Combining spectral data and the results of reference analyses, partial least squares (PLS) models were constructed and validated. The relative standard errors of prediction (RSEP) were calculated for the calibration and validation sets. Regarding macronutrient analysis, the latter were found to be in the 1.7-2.3%, 2.5-3.6% and 2.6-3.3% ranges for protein, reducing sugars and fat determination, respectively. These errors amounted to 3.1-3.5% for TPs and 2.2-3.4% for antioxidant activity quantification, respectively, whereas they were found to be in the 1.5-2.3% and 1.6-3.2% ranges for pH and the color parameters' determination.

摘要

振动光谱学被应用于测定花粉中的宏量营养素水平、总多酚（TPs）含量、抗氧化活性、pH 值和颜色参数。对均质花粉颗粒记录了中红外和近红外区域的拉曼、衰减全反射和漫反射光谱。结合光谱数据和参考分析结果，构建并验证了偏最小二乘（PLS）模型。为校准集和验证集计算了相对标准误差预测值（RSEP）。对于宏量营养素分析，蛋白质、还原糖和脂肪测定的 RSEP 值分别在 1.7-2.3%、2.5-3.6%和 2.6-3.3%范围内。TPs 的 RSEP 值为 3.1-3.5%，抗氧化活性的定量 RSEP 值为 2.2-3.4%，而 pH 值和颜色参数测定的 RSEP 值分别在 1.5-2.3%和 1.6-3.2%范围内。